In this study, scallop mantle protein was treated by ultrasound at different powers, and then analyzed by ANS fluorescent probes, circular dichroism spectroscopy, endogenous fluorescence spectrum, DNTB colorimetry and in-vitro digestion model to elucidate the structure–function relationship. The results indicated that ultrasound can significantly affect the secondary structure of scallop mantle protein like enhancing hydrophobicity, lowering the particle size, increasing the relative contents of α-helix and decreasing contents of β-pleated sheet, β-turn and random coil, as well as altering intrinsic fluorescence intensity with blue shift of maximum fluorescence peak. But ultrasound had no effect on its primary structure. Moreover, the functions of scallop mantle protein were regulated by modifying its structures by ultrasound. Specifically, the protein had the highest performance in foaming property and in-vitro digestibility under ultrasonic power of 100 W, oil binding capacity under 100 W, water binding capacity under 300 W, solubility and emulsification capacity under 400 W, and emulsion stability under 600 W. These results prove ultrasonic treatment has the potential to effectively improve functional properties and quality of scallop mantle protein, benefiting in comprehensive utilization of scallop mantles.

本研究采用不同功率的超声处理扇贝外套膜蛋白，然后通过ANS荧光探针、圆二色光谱、内源荧光光谱、DNTB比色法和体外消化模型进行分析，以阐明其结构与功能关系。结果表明，超声可显着影响扇贝外套膜蛋白的二级结构，增强疏水性，降低粒径，增加α螺旋相对含量，降低β折叠片、β转角和无规卷曲含量。随着最大荧光峰蓝移改变固有荧光强度。但超声波对其一级结构没有影响。此外，扇贝外套膜蛋白的功能是通过超声改变其结构来调节的。具体而言，该蛋白在超声波功率100W下具有最高的起泡性和体外消化率，在100W下具有最高的油结合能力，在300W下具有最高的水结合能力，在400W下具有最高的溶解度和乳化能力，在600W下具有最高的乳化稳定性。 W.这些结果证明超声波处理有可能有效改善扇贝套膜蛋白的功能特性和质量，有利于扇贝套膜的综合利用。