Abstract

Chlorpyrifos is an organophosphate and the cypermethrin is type 2 pyrethroid insecticide that are used for indoor and outdoor pest control. The present study aimed to investigate differential transcriptional profiling to identify the candidate gene associated with lung injury following exposure to chlorpyrifos and/or cypermethrin in a mouse model system. Swiss male albino mice (n = 24) were divided into three treatment groups (n = 6 each) that were given chlorpyrifos (2.76 mg kg⁻¹ body weight), cypermethrin (2 mg kg⁻¹ body weight) and the combination of both pesticides orally dissolved in corn oil and one control group (n = 6) that received corn oil for 90 days. The pulmonary expression of the Apaf1 was observed using RT2 Profiler PCR Array. The results showed that chronic exposure to chlorpyrifos, cypermethrin and their combination downregulated (67, 63 and 66 genes) and upregulated (4, 2 and 2 genes), respectively. The pulmonary expression of Apaf1 that plays important role in apoptosis was found to be downregulated. The immunohistochemistry depicted reduced expression of Apaf1 in both airway epithelium and alveolar septa following exposure to chlorpyrifos and/or cypermethrin. In conclusion, results demonstrated that exposure to chlorpyrifos, cypermethrin and their combination cause lung damage by the dysregulation of Apaf1 gene expression.

摘要

毒死蜱是一种有机磷酸酯，氯氰菊酯是 2 型拟除虫菊酯杀虫剂，用于室内和室外害虫防治。本研究旨在研究差异转录谱，以确定与小鼠模型系统中接触毒死蜱和/或氯氰菊酯后肺损伤相关的候选基因。瑞士雄性白化小鼠（n  = 24）被分为三个治疗组（每组n  = 6），分别给予毒死蜱（2.76 mg kg ^-1体重）、氯氰菊酯（2 mg kg ^-1体重）以及两者的组合口服溶解在玉米油中的农药和一组对照组（n = 6) 接受玉米油 90 天。使用 RT2 Profiler PCR Array 观察 Apaf1 的肺部表达。结果表明，长期接触毒死蜱、氯氰菊酯及其组合分别下调（67、63和66个基因）和上调（4、2和2个基因）。发现在细胞凋亡中起重要作用的 Apaf1 的肺部表达下调。免疫组织化学显示，接触毒死蜱和/或氯氰菊酯后，气道上皮和肺泡间隔中 Apaf1 的表达均减少。总之，结果表明，接触毒死蜱、氯氰菊酯及其组合会因 Apaf1 基因表达失调而导致肺损伤。