Abstract

Accurate and rapid determination of bacterial disease agents of fish is an important step for sustainable and efficient aquaculture production. In general, biochemical and molecular methods are used for pathogen detection but they are usually time-consuming and required qualified personnel. Recently spectroscopic methods are preferred in clinical and food microbiology and declared as a promising alternative method for pathogens diagnosis with many advantages. In this study, the significant spectra of three important bacterial fish pathogens (Lactococcus garvieae, Vibrio anguillarum and Yersinia ruckeri) were determined by Raman spectroscopy. The first data of the pathogens were obtained and the distinctive differences in polysaccharides, nucleic acids, fatty acids and amino acids were identified. This preliminary study aimed to be pioneer for further studies in aquaculture and veterinary microbiology toward developing an alternative method for routine identification.

摘要

准确快速测定鱼类细菌性病原是水产养殖可持续高效生产的重要一步。一般来说，生化和分子方法用于病原体检测，但通常耗时且需要合格的人员。最近，光谱方法在临床和食品微生物学中受到青睐，并被认为是一种有前途的病原体诊断替代方法，具有许多优点。在这项研究中，三种重要的细菌性鱼类病原体（加维乳球菌、鳗弧菌和鲁氏耶尔森氏菌）的显着谱）通过拉曼光谱测定。获得了病原体的第一批数据，并鉴定了多糖、核酸、脂肪酸和氨基酸的显着差异。这项初步研究旨在成为水产养殖和兽医微生物学进一步研究的先驱，以开发一种常规鉴定的替代方法。