Hyperglycaemia damages the microvasculature in part through the reduced recruitment of immune cells and interference with platelet signalling, leading to poor wound healing and accelerated lipid deposition in mammals. We investigated the utility of zebrafish larvae to model the effect of exogenous glucose on neutrophil and macrophage recruitment to a tail wound, wound-induced haemostasis, and chicken egg yolk feed challenge-induced hyperlipidaemia by supplementing larvae with exogenous glucose by immersion or injection. Neither method of glucose supplementation affected the recruitment of neutrophils and macrophages following tail transection. Glucose injection reduced thrombocyte retention and fibrin plug formation while only thrombocyte retention was reduced by glucose immersion following tail transection. We observed accelerated lipid accumulation in glucose-injected larvae challenged with high fat chicken egg yolk feeding. Our study identifies conserved and divergent effects of high glucose on inflammation, haemostasis, and hyperlipidaemia in zebrafish larvae compared to mammals.

高血糖在一定程度上通过减少免疫细胞的募集和干扰血小板信号传导来损害微脉管系统，导致哺乳动物伤口愈合不良并加速脂质沉积。我们研究了斑马鱼幼虫的用途，通过浸泡或注射向幼虫补充外源葡萄糖，以模拟外源葡萄糖对中性粒细胞和巨噬细胞募集到尾部伤口、伤口诱导的止血和鸡蛋黄饲料攻击诱导的高脂血症的影响。补充葡萄糖的方法都不影响尾部横断后中性粒细胞和巨噬细胞的募集。注射葡萄糖减少了血小板滞留和纤维蛋白栓的形成，而尾部横断后的葡萄糖浸泡仅减少了血小板滞留。我们观察到，在接受高脂肪鸡蛋黄喂养的注射葡萄糖的幼虫中，脂质积累加速。我们的研究确定了与哺乳动物相比，高葡萄糖对斑马鱼幼虫的炎症、止血和高脂血症的保守和不同的影响。