We combined traditional mRNA-seq and RNC-seq together to reveal post-transcriptional regulation events impacting gene expression and interactions between the serious fungal pathogen Verticillium dahliae and a susceptible host, Gossypium hirsutum TM-1. After screening the differentially expressed and translated genes, V. dahliae infection was observed to influence gene transcription and translation in its host. Interestingly, the asparagine synthase (ASN1) gene transcripts increased significantly with the increase of infection time, while the rate of ASN1 protein accumulation in host TM-1 was distinctly lower than that in resistant hosts. We knocked down the ASN1 gene in resistant plants (ZZM2), and found that Verticillium-resistance was significantly reduced upon knockdown of ASN1. Our study revealed both transcriptional and post-transcriptional regulation of gene expression in TM-1 cotton plants infected by V. dahliae, and showed that ASN1 functions in the V. dahliae resistance process. These insights support breeding of disease resistance in cotton.

我们将传统的 mRNA-seq 和 RNC-seq 结合在一起，揭示了影响基因表达的转录后调控事件以及严重真菌病原体大丽轮枝菌与易感宿主Gossypium hirsutum TM-1 之间的相互作用。在筛选差异表达和翻译的基因后，观察到大丽花感染会影响其宿主中的基因转录和翻译。有趣的是，天冬酰胺合酶（ASN1）基因转录本随着感染时间的增加而显着增加，而宿主TM-1中ASN1蛋白的积累率明显低于抗性宿主。我们击倒了ASN1抗性植物（ZZM2）中的基因，发现在敲低ASN1后，黄萎病菌的抗性显着降低。我们的研究揭示了大丽花感染的 TM-1 棉花植物中基因表达的转录和转录后调控，并表明ASN1在大丽花抗性过程中发挥作用。这些见解支持在棉花中培育抗病性。