Chimeric antigen receptor T cell (CAR T) therapy reached a milestone in the treatment of relapsed and refractory B-cell malignancies. However, beneficial effects of CAR T cells have not been obtained in solid tumors yet. Herein, we implemented a porous microneedle patch that accommodates CAR T cells and allows in situ penetration-mediated seeding of CAR T cells when implanted in the tumor bed or in post-surgical resection cavity. CAR T cells loaded in the pores of the microneedle tips were readily escorted to the tumor in an evenly scattered manner without losing their activity. Such microneedle-mediated local delivery enhanced infiltration and immunostimulation of CAR T cells as compared to direct intratumoral injection. This tailorable patch offers a transformative platform for scattered seeding of living cells for treating a variety of tumors.

中文翻译：

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在实体瘤中分散接种 CAR T 细胞可增强抗癌功​​效

嵌合抗原受体 T 细胞 (CAR T) 疗法在治疗复发性和难治性 B 细胞恶性肿瘤方面达到了一个里程碑。然而，CAR T 细胞的有益作用尚未在实体瘤中获得。在这里，我们实施了一种多孔微针贴片，该贴片可容纳 CAR T 细胞，并在植入肿瘤床或手术后切除腔时允许 CAR T 细胞的原位穿透介导播种。装载在微针尖端孔隙中的CAR T细胞很容易以均匀分散的方式被护送到肿瘤处，而不会失去它们的活性。与直接肿瘤内注射相比，这种微针介导的局部递送增强了 CAR T 细胞的浸润和免疫刺激。这种可定制的贴片为分散接种活细胞以治疗各种肿瘤提供了一个变革性平台。