Real-time imaging of cell-surface proteins with antibody-based fluorogenic probes,Chemical Science

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Real-time imaging of cell-surface proteins with antibody-based fluorogenic probes
Chemical Science ( IF 7.6 ) Pub Date : 2021-09-16 , DOI: 10.1039/d1sc03065e
Wenchao Wang ₁ , Ying Zhang ₁ , Hong Zhao ₁ , Xinlei Zhuang ₁ , Haoting Wang ₁ , Kaifeng He _{1,

2} , Wanting Xu ₁ , Yu Kang ₁ , Shuqing Chen ₁ , Su Zeng _{1,

2} , Linghui Qian _{1,

2}

Affiliation

Cell-surface proteins, working as key agents in various diseases, are the targets for around 66% of approved human drugs. A general strategy to selectively detect these proteins in a real-time manner is expected to facilitate the development of new drugs and medical diagnoses. Although brilliant successes were attained using small-molecule probes, they could cover a narrow range of targets due to the lack of suitable ligands and some of them suffer from selectivity issues. We report herein an antibody-based fluorogenic probe prepared via a two-step chemical modification under physiological conditions, to fulfill the selective recognition and wash-free imaging of membrane proteins, establishing a modular strategy with broad implications for biochemical research and for therapeutics.

中文翻译：

使用基于抗体的荧光探针对细胞表面蛋白进行实时成像

细胞表面蛋白是多种疾病的关键因子，是约 66% 已批准人类药物的靶标。实时选择性检测这些蛋白质的总体策略有望促进新药和医学诊断的开发。尽管使用小分子探针取得了辉煌的成功，但由于缺乏合适的配体，它们只能覆盖狭窄的靶标范围，而且其中一些探针还存在选择性问题。我们在此报道了一种在生理条件下通过两步化学修饰制备的基于抗体的荧光探针，以实现膜蛋白的选择性识别和免洗成像，建立了对生化研究和治疗具有广泛影响的模块化策略。

更新日期：2021-09-24

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