Towards a native environment: structure and function of membrane proteins in lipid bilayers by NMR,Chemical Science

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Towards a native environment: structure and function of membrane proteins in lipid bilayers by NMR
Chemical Science ( IF 7.6 ) Pub Date : 2021-09-07 , DOI: 10.1039/d1sc02813h
Kai Xue ₁ , Kumar Tekwani Movellan ₁ , Xizhou Cecily Zhang ₁ , Eszter E Najbauer ₁ , Marcel C Forster ₁ , Stefan Becker ₁ , Loren B Andreas ₁

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Solid-state NMR (ssNMR) is a versatile technique that can be used for the characterization of various materials, ranging from small molecules to biological samples, including membrane proteins. ssNMR can probe both the structure and dynamics of membrane proteins, revealing protein function in a near-native lipid bilayer environment. The main limitation of the method is spectral resolution and sensitivity, however recent developments in ssNMR hardware, including the commercialization of 28 T magnets (1.2 GHz proton frequency) and ultrafast MAS spinning (<100 kHz) promise to accelerate acquisition, while reducing sample requirement, both of which are critical to membrane protein studies. Here, we review recent advances in ssNMR methodology used for structure determination of membrane proteins in native and mimetic environments, as well as the study of protein functions such as protein dynamics, and interactions with ligands, lipids and cholesterol.

中文翻译：

走向天然环境：核磁共振脂质双层膜蛋白的结构和功能

固态核磁共振 (ssNMR) 是一种通用技术，可用于表征各种材料，从小分子到生物样品，包括膜蛋白。ssNMR 可以探测膜蛋白的结构和动力学，揭示近乎天然的脂质双层环境中的蛋白质功能。该方法的主要限制是光谱分辨率和灵敏度，但是 ssNMR 硬件的最新发展，包括 28 T 磁体（1.2 GHz 质子频率）和超快 MAS 旋转（<100 kHz）的商业化有望加速采集，同时减少样品需求，这两者对于膜蛋白研究都至关重要。在这里，我们回顾了用于在天然和模拟环境中确定膜蛋白结构的 ssNMR 方法的最新进展，

更新日期：2021-09-24

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