Background: The Red Sea sponges have been endorsed as a plentiful source of bioactive compounds with promising anti-cancer and anti-inflammatory activities; therefore, exploring their potential as a source of anti-cancer metabolites has stimulated a growing research interest. Purpose: To investigate the anti-cancer and anti-inflammatory potential of the Red Sea sponges, in their bulk and silver nanostructure. Metabolomics analysis of the selected sponge followed by molecular docking studies, will be conducted to explore and predict the secondary metabolites that might provide its capability of inhibiting cancer. Materials and Methods: We prepared a chloroform extract (CE) and ethyl acetate extract (EE) of the Red Sea sponge Phyllospongia lamellosa synthesized silver nanoparticles. The prepared silver nanoparticles were characterized through UV–vis spectrophotometric, transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR) analyses. Testing for their anti-cancer activities was performed against MCF-7, MDB-231, and MCF-10A cells. Anti-inflammatory activity against COX-1 and 2 was assessed. Furthermore, liquid chromatography–mass spectrometry (LC–MS)-based metabolomics analysis and molecular docking were also applied.

中文翻译：

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代谢组学分析和对接研究支持的红海海绵叶海绵绿色合成银纳米颗粒的抗癌和抗炎潜力

背景：红海海绵已被认可为具有良好抗癌和抗炎活性的生物活性化合物的丰富来源；因此，探索它们作为抗癌代谢物来源的潜力已经激发了越来越多的研究兴趣。目的：研究红海海绵的块状和银纳米结构的抗癌和抗炎潜力。将对选定的海绵进行代谢组学分析，然后进行分子对接研究，以探索和预测可能提供其抑制癌症能力的次级代谢物。材料和方法：我们制备了红海海绵Phyllospongia lamellosa的氯仿提取物 (CE) 和乙酸乙酯提取物 (EE)合成银纳米粒子。通过紫外-可见分光光度法、透射电子显微镜 (TEM) 和傅里叶变换红外光谱 (FTIR) 分析对制备的银纳米粒子进行表征。针对 MCF-7、MDB-231 和 MCF-10A 细胞对其抗癌活性进行了测试。评估了针对 COX-1 和 2 的抗炎活性。此外，还应用了基于液相色谱-质谱 (LC-MS) 的代谢组学分析和分子对接。