Leukocytes offer a critical layer of protection to the host following skin infections. Delineating the kinetics of cutaneous leukocyte recruitment as well as their anti-microbial and regulatory profiles is challenging since it requires the isolation of adequate cell numbers and maintenance of their functional properties. Herein, we took advantage of a modified procedure to gain insights into the contributions of fish phagocytes through induction and resolution phases of acute cutaneous inflammation in goldfish (Carassius auratus). Our data shows early upregulation of pro-inflammatory cytokines and chemokines, which was paired with neutrophil-dominant leukocyte migration of neutrophils from circulation to the injury site. Recruited neutrophils were associated with high levels of reactive oxygen species (ROS). Following pathogen elimination, a reduction in ROS levels and pro-inflammatory cytokines expression preceded the resolution of inflammation. These results provide a better understanding of the cutaneous immune responses in fish. Moreover, the increased viability and functionality of isolated skin leukocytes opens the door to better understand a range of additional skin diseases.

白细胞在皮肤感染后为宿主提供了关键的保护层。描绘皮肤白细胞募集的动力学以及它们的抗菌和调节特性具有挑战性，因为它需要分离足够的细胞数量并维持其功能特性。在此，我们利用修改后的程序，通过金鱼急性皮肤炎症的诱导和消退阶段深入了解鱼类吞噬细胞的作用。鲫鱼）。我们的数据显示促炎细胞因子和趋化因子的早期上调，这与嗜中性粒细胞为主的白细胞从循环到损伤部位的迁移配对。招募的中性粒细胞与高水平的活性氧 (ROS) 相关。在消除病原体后，ROS 水平和促炎细胞因子表达的降低先于炎症消退。这些结果提供了对鱼类皮肤免疫反应的更好理解。此外，分离的皮肤白细胞的活力和功能的增加为更好地了解一系列其他皮肤病打开了大门。