Optical imaging has rapidly evolved in the last decades. Sophisticated microscopes allowing optical sectioning for 3D imaging or sub-diffraction resolution are available. Due to price and maintenance issues, these microscopes are often shared between users in facilities. Consequently, long term access is often prohibited and does not allow to monitor slowly evolving biological systems or to validate new models like organoids. Preliminary coarse long-term data that do not require acquisition of terabytes of high-resolution images are important as a first step. By contrast with expansive all-in-one commercialized stations, standard microscopes equipped with incubator stages offer a more cost-effective solution despite imperfect long run atmosphere and temperature control.

中文翻译：

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Incubascope：一种简单、紧凑且大视野的显微镜，用于在培养箱内进行长期成像

在过去的几十年中，光学成像发展迅速。可提供用于 3D 成像或亚衍射分辨率的光学切片的精密显微镜。由于价格和维护问题，这些显微镜通常在设施中的用户之间共享。因此，长期访问通常是被禁止的，并且不允许监测缓慢进化的生物系统或验证类器官等新模型。作为第一步，不需要获取 TB 级高分辨率图像的初步粗略长期数据很重要。与庞大的一体化商业化工作站相比，配备孵化台的标准显微镜提供了更具成本效益的解决方案，尽管长期运行环境和温度控制不完善。