Acoustic Droplet Printing Tumor Organoids for Modeling Bladder Tumor Immune Microenvironment within a Week,Advanced Healthcare Materials

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Acoustic Droplet Printing Tumor Organoids for Modeling Bladder Tumor Immune Microenvironment within a Week
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-09-23 , DOI: 10.1002/adhm.202101312
Zhiyi Gong ₁ , Lanxiang Huang _{2,

3} , Xuan Tang _{2,

3} , Keke Chen ₁ , Zhuhao Wu ₁ , Lingling Zhang ₂ , Yue Sun ₁ , Yu Xia ₁ , Hui Chen ₁ , Yongchang Wei ₄ , Fubing Wang _{2,

3} , Shishang Guo ₁

Affiliation

Current organoid models are limited by the incapability of rapidly fabricating organoids that can mimic the immune microenvironment for a short term. Here, an acoustic droplet-based platform is presented to facilitate the rapid formation of tumor organoids, which retains the original tumor immune microenvironment and establishes a personalized bladder cancer tumor immunotherapy model. In combination with a hydrophobic substrate, the acoustic droplet printer can yield a large number of homogeneous and highly viable bladder tumor organoids in vitro within a week. The generated organoids consist of all components of bladder tumor, including diverse immune elements and tumor cells. By coculturing tumor organoids with autologous immune cells for 2 days, tumor reactive T cells are induced in vitro. Furthermore, it is also demonstrated that these tumor-reactive T cells can also enhance the killing efficiency of matched organoids. Because of the easy operation, repeatability, and stability, the proposed acoustic droplet platform will provide a reliable approach for personalized tumor immunotherapy.

中文翻译：

用于在一周内模拟膀胱肿瘤免疫微环境的声学液滴打印肿瘤类器官

当前的类器官模型受限于无法快速制造能够在短期内模拟免疫微环境的类器官。在这里，提出了一种基于声学液滴的平台，以促进肿瘤类器官的快速形成，该平台保留了原有的肿瘤免疫微环境，并建立了个性化的膀胱癌肿瘤免疫治疗模型。结合疏水性基质，声学液滴打印机可以在一周内在体外产生大量均质且高度可行的膀胱肿瘤类器官。生成的类器官由膀胱肿瘤的所有成分组成，包括多种免疫元件和肿瘤细胞。通过将肿瘤类器官与自体免疫细胞共培养 2 天，在体外诱导肿瘤反应性 T 细胞。此外，还证明了这些肿瘤反应性 T 细胞还可以提高匹配的类器官的杀伤效率。由于易于操作、可重复性和稳定性，所提出的声学液滴平台将为个性化肿瘤免疫治疗提供可靠的方法。

更新日期：2021-11-17

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