Background

Until a few years ago, keratoconus was defined as a noninflammatory degenerative disease. However, recent studies have shown that the altered balance between inflammatory cytokines, proteases, and protease inhibitors, as well as free radicals and oxidants, have a crucial role in the pathogenesis of this disease. The aim of this study is to investigate whether interleukin 17 A G197A (rs2275913) and interleukin 17 F T7488C (rs763780) polymorphisms are associated with keratoconus in patients from a population of the northwestern region of the State of São Paulo, Brazil.

Methods and Results

35 patients and 61 controls were enrolled. Genotyping of interleukin 17 A G197A and interleukin 17 F T7488C polymorphisms was carried out using the polymerase chain reaction-restriction fragment length polymorphism technique. Statistical analyses were conducted using the chi-square test, and an odds ratio with a 95% confidence interval was also calculated to evaluate the association between polymorphisms and disease. Evaluating interleukin 17 F T7488C, we found that the TT genotype is associated as a risk factor for keratoconus (P = 0.04; OR = 3.01; CI 1.11–8.14). As for evaluating interleukin 17 A G197A, the allele and genotype frequencies between patients and controls were compared and no statistically significant differences were found.

Conclusions

Our data showed that the interleukin 17 F T7488C polymorphisms may exert an influence in keratoconus.

中文翻译：

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白细胞介素 17 A 和 17 F 多态性对圆锥角膜的影响

背景

直到几年前，圆锥角膜还被定义为一种非炎症性退行性疾病。然而，最近的研究表明，炎症细胞因子、蛋白酶和蛋白酶抑制剂以及自由基和氧化剂之间的平衡改变在这种疾病的发病机制中起着至关重要的作用。本研究的目的是调查白细胞介素17 A G197A (rs2275913) 和白细胞介素17 F T7488C (rs763780) 多态性是否与巴西圣保罗州西北部地区患者的圆锥角膜相关。

方法和结果

招募了 35 名患者和 61 名对照。使用聚合酶链反应-限制性片段长度多态性技术对白细胞介素17 A G197A 和白细胞介素17 F T7488C 多态性进行基因分型。使用卡方检验进行统计分析，并计算具有95％置信区间的优势比以评估多态性与疾病之间的关联。评估白细胞介素17 F T7488C，我们发现 TT 基因型与圆锥角膜的危险因素相关（P  = 0.04；OR = 3.01；CI 1.11-8.14）。至于评估白介素17 AG197A，比较患者和对照组的等位基因和基因型频率，没有发现统计学上的显着差异。

结论

我们的数据显示白细胞介素17 F T7488C 多态性可能对圆锥角膜产生影响。