The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p<0.0001 (treatment v/s control group). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-Formazan (MTT) assay results showed that treatment had better survival rates than the control group at different time intervals i.e. 48 h, and 72 h with p<0.01. Mechanosensation (behavioral study) as well as in vivo study results showed that at 0 h, 10 μg/ml of DAM-1 treatment showed a better anti-oxidative activity than the control group, 25 and 50 μg/ml of DAM-1 treated groups with p<0.001 but at 2.5 h incubation with 10, 25, 50 μg/ml of DAM-1 showed an increased anti-oxidative activity than the control group with p<0.001. Thermoresistance assay confirmed that the treatment group had more survival than control group with p<0.001. Absorption study of DAM-1 in C. elegans has shown that the absorption of the drug increases up to 180 mins with a slight decrease after 360 mins and then constant absorption up to 1440 mins. This study paves the way towards the initiative to explore the pharmacological role of DAM-1 in various oxidative stress mediated diseases at molecular levels and the absorption study points out its potential role which could be utilized in the metabolomics and proteomics analysis of this compound in other studies.

中文翻译：

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使用秀丽隐杆线虫对从 Dolichandrone atrovirens 中提取的天然化合物作为新型抗氧化剂进行评估。


从 Dolichandrone atrovirens 的甲醇提取物中分离得到化合物甲基肉桂酰梓醇 (DAM-1)。研究已经报道了 Dolichandrone atrovirens 树皮提取物的抗氧化活性，但迄今为止，分离化合物 DAM-1 的抗氧化活性仍未得到探索。活性氧生成的内源过程会导致各种退行性疾病，可以使用植物来源的这些外源部分来分解，在本研究中，我们试图使用秀丽隐杆线虫（C. elegans）评估 DAM-1 化合物的抗氧化潜力），这是研究化合物抗氧化活性的主要模型。细胞毒性测定结果显示，浓度为 10、25 和 50 μg/ml 的 DAM-1 处理分别显示出 100%、91% 和 50% 的存活率，总体 p<0.0001（处理组与对照组）。 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑-甲臜(MTT)检测结果显示，治疗组在不同时间间隔（48小时、72小时）的存活率均优于对照组。 p<0.01。机械感觉（行为研究）以及体内研究结果表明，在 0 小时时，10 μg/ml 的 DAM-1 处理组比对照组（25 和 50 μg/ml 的 DAM-1 处理组）表现出更好的抗氧化活性。具有p<0.001的组但在与10、25、50μg/ml的DAM-1孵育2.5小时时，显示出比具有p<0.001的对照组增加的抗氧化活性。耐热性测定证实治疗组的存活率高于对照组，p<0.001。 DAM-1 在秀丽隐杆线虫中的吸收研究表明，药物的吸收在 180 分钟内增加，360 分钟后略有下降，然后持续吸收直至 1440 分钟。 这项研究为在分子水平上探索 DAM-1 在各种氧化应激介导的疾病中的药理作用铺平了道路，吸收研究指出了其潜在作用，可用于该化合物在其他疾病中的代谢组学和蛋白质组学分析。研究。