The compound methyl cinnamoyl catalpol (DAM-1) was isolated from the methanol extract of Dolichandrone atrovirens. Studies have already reported the antioxidant activity of Dolichandrone atrovirens bark extract, but till date the antioxidant activity of the isolated compound DAM-1, remains unexplored. The endogenous process of reactive oxygen species generation which leads to various degenerative diseases, can be broken down using these exogenous moieties from plant origin, herein this study we sought to evaluate the antioxidant potential of the DAM-1 compound using Caenorhabditis elegans (C. elegans), which is the primary model to study the antioxidant activity of compounds. Cytotoxicity assay results showed that DAM-1 treatment in the concentration of 10, 25 and 50 μg/ml has shown 100%, 91%, and 50% survival respectively with overall p<0.0001 (treatment v/s control group). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-Formazan (MTT) assay results showed that treatment had better survival rates than the control group at different time intervals i.e. 48 h, and 72 h with p<0.01. Mechanosensation (behavioral study) as well as in vivo study results showed that at 0 h, 10 μg/ml of DAM-1 treatment showed a better anti-oxidative activity than the control group, 25 and 50 μg/ml of DAM-1 treated groups with p<0.001 but at 2.5 h incubation with 10, 25, 50 μg/ml of DAM-1 showed an increased anti-oxidative activity than the control group with p<0.001. Thermoresistance assay confirmed that the treatment group had more survival than control group with p<0.001. Absorption study of DAM-1 in C. elegans has shown that the absorption of the drug increases up to 180 mins with a slight decrease after 360 mins and then constant absorption up to 1440 mins. This study paves the way towards the initiative to explore the pharmacological role of DAM-1 in various oxidative stress mediated diseases at molecular levels and the absorption study points out its potential role which could be utilized in the metabolomics and proteomics analysis of this compound in other studies.

中文翻译：

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使用秀丽隐杆线虫评估从 Dolichandrone atrovirens 中提取的天然化合物作为新型抗氧化剂。

化合物甲基肉桂酰梓醇 (DAM-1) 是从 Dolichandrone atrovirens 的甲醇提取物中分离出来的。研究已经报道了 Dolichandrone atrovirens 树皮提取物的抗氧化活性，但迄今为止，分离的化合物 DAM-1 的抗氧化活性仍未得到探索。可以使用这些植物来源的外源部分来分解导致各种退行性疾病的活性氧产生的内源性过程，在此研究中，我们试图使用秀丽隐杆线虫（秀丽隐杆线虫）评估 DAM-1 化合物的抗氧化潜力)，这是研究化合物抗氧化活性的主要模型。细胞毒性测定结果显示，10、25 和 50 μg/ml 浓度的 DAM-1 处理分别显示 100%、91% 和 50% 的存活率，总体 p<0。0001（治疗组与对照组）。3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物-甲臜(MTT)测定结果显示，治疗组在不同时间间隔即48小时和72小时的存活率均优于对照组。 p<0.01。机械感觉（行为研究）以及体内研究结果表明，在 0 h 时，10 μg/ml DAM-1 处理显示出比对照组、25 和 50 μg/ml DAM-1 处理更好的抗氧化活性p<0.001 但在与 10、25、50 μg/ml DAM-1 孵育 2.5 小时后，与 p<0.001 的对照组相比，抗氧化活性增强。耐热试验证实，治疗组比对照组有更多的存活率，p<0.001。DAM-1 在 C 中的吸收研究。elegans 表明，药物的吸收增加到 180 分钟，360 分钟后略有下降，然后持续吸收到 1440 分钟。这项研究为主动探索 DAM-1 在分子水平上的各种氧化应激介导的疾病中的药理作用铺平了道路，吸收研究指出了其潜在作用，可用于该化合物在其他方面的代谢组学和蛋白质组学分析。学习。