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Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere.
PLOS ONE ( IF 2.9 ) Pub Date : 2021-09-22 , DOI: 10.1371/journal.pone.0257017
Alexa K Hoke 1 , Guadalupe Reynoso 1, 2 , Morgan R Smith 1, 3 , Malia I Gardner 1 , Dominique J Lockwood 1 , Naomi E Gilbert 1, 4 , Steven W Wilhelm 4 , Isabella R Becker 1 , Grant J Brennan 1 , Katherine E Crider 1 , Shannon R Farnan 1 , Victoria Mendoza 1 , Alison C Poole 1 , Zachary P Zimmerman 1 , Lucy K Utz 1 , Louie L Wurch 1 , Morgan M Steffen 1
Affiliation  

Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in the western basin of Lake Erie. The genomes of five isolates (Exiguobacterium sp. JMULE1, Enterobacter sp. JMULE2, Deinococcus sp. JMULE3, Paenibacillus sp. JMULE4, and Acidovorax sp. JMULE5.) were sequenced on a PacBio Sequel system. These genomes ranged in size from 3.1 Mbp (Exiguobacterium sp. JMULE1) to 5.7 Mbp (Enterobacter sp. JMULE2). The genomes were analyzed for genes relating to critical metabolic functions, including nitrogen reduction and carbon utilization. All five of the sequenced genomes contained genes that could be used in potential signaling and nutrient exchange between the bacteria and cyanobacteria such as Microcystis. Gene expression signatures of algal-derived carbon utilization for two isolates were identified in Microcystis blooms in Lake Erie and Lake Tai (Taihu) at low levels, suggesting these organisms are active and may have a functional role during Microcystis blooms in aggregates, but were largely missing from whole water samples. These findings build on the growing evidence that the bacterial microbiome associated with bloom-forming algae have the functional potential to contribute to nutrient exchange within bloom communities and interact with important bloom formers like Microcystis.

中文翻译:

伊利湖细菌的基因组特征表明微囊藻藻类中存在相互作用。

已经在海洋系统中检查了有害藻华 (HAB) 群落中的微生物相互作用,但在淡水中的研究很少。为了研究 HAB 与微生物的相互作用,我们在 2017 年伊利湖西部盆地爆发期间分离了与形成藻华的蓝藻微囊藻属密切相关的细菌。五个分离株(Exiguobacterium sp. JMULE1、Enterobacter sp. JMULE2、Deinococcus sp. JMULE3、Paenibacillus sp. JMULE4 和 Acidovorax sp. JMULE5.)的基因组在 PacBio Sequel 系统上进行了测序。这些基因组的大小范围从 3.1 Mbp (Exiguobacterium sp. JMULE1) 到 5.7 Mbp (Enterobacter sp. JMULE2)。分析了基因组中与关键代谢功能相关的基因,包括氮还原和碳利用。所有五个测序的基因组都包含可用于细菌和蓝藻(如微囊藻)之间的潜在信号传导和营养交换的基因。在伊利湖和太湖(太湖)的微囊藻水华中发现了两种分离株藻源性碳利用的基因表达特征,表明这些生物体是活跃的,可能在微囊藻聚集期间发挥功能作用,但在很大程度上是整个水样中缺失。这些发现建立在越来越多的证据基础上,即与形成水华的藻类相关的细菌微生物群具有促进水华群落内营养交换的功能潜力,并与微囊藻等重要的水华形成者相互作用。在伊利湖和太湖(太湖)的微囊藻水华中发现了两种分离株藻源性碳利用的基因表达特征,表明这些生物体是活跃的,可能在微囊藻聚集期间发挥功能作用,但在很大程度上是整个水样中缺失。这些发现建立在越来越多的证据基础上,即与形成水华的藻类相关的细菌微生物群具有促进水华群落内营养交换的功能潜力,并与微囊藻等重要的水华形成者相互作用。在伊利湖和太湖(太湖)的微囊藻水华中发现了两种分离株藻源性碳利用的基因表达特征,表明这些生物体是活跃的,可能在微囊藻聚集期间发挥功能作用,但在很大程度上是整个水样中缺失。这些发现建立在越来越多的证据基础上,即与形成水华的藻类相关的细菌微生物群具有促进水华群落内营养交换的功能潜力,并与微囊藻等重要的水华形成者相互作用。但在整个水样中基本缺失。这些发现建立在越来越多的证据基础上,即与形成水华的藻类相关的细菌微生物群具有促进水华群落内营养交换的功能潜力,并与微囊藻等重要的水华形成者相互作用。但在整个水样中基本缺失。这些发现建立在越来越多的证据基础上,即与形成水华的藻类相关的细菌微生物群具有促进水华群落内营养交换的功能潜力,并与微囊藻等重要的水华形成者相互作用。
更新日期:2021-09-22
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