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Mapping the major quantitative trait loci of the heading date trait in Qingke barley (Hordeum vulgare L.) from the Qinghai-Tibetan Plateau via genotyping by sequencing
Frontiers in Life Science Pub Date : 2021-09-22 , DOI: 10.1080/26895293.2021.1980439
Xin Li 1, 2, 3, 4 , Xinlian Yu 1, 2, 3, 4 , Xiaohua Yao 1, 2, 3, 4 , Youhua Yao 1, 2, 3, 4 , Yixiong Bai 1, 2, 3, 4 , Likun An 1, 2, 3, 4 , Kunlun Wu 1, 2, 3, 4
Affiliation  

A key trait impacting the maturity of qingke is the heading date. Identification of major stable quantitative trait loci (QTL) for heading date trait is important for maturity improvement in qingke. Therefore, we conducted QTL mapping of an F2 population of 146 lines and constructed a high-density linkage map with 1,243 recombination bin markers. After phenotyping the F2:3 across three environments to detect QTL for heading date. The results showed that three QTLs that play a major role in heading date were identified, cqHD2H-1 and cqHD2H-2 were identified across two environments and considered environmentally stable. The cqHD2H-2, reflects a stable early-heading locus, explaining 27.50% of the phenotypic variance. Mapping of the cqHD2H-2 was subsequently conducted in extremely heading individuals from F5 populations, the cqHD2H-2 was further delimited to a region of approximately 84 Mb on 2H, and the HORVU2Hr1G087460 gene was identified as a homologue of the rice DTH8 gene by a BLASTN analysis of the candidate region. These results broaden our understanding on genetic basis of heading date and provide clues for fine mapping and marker-assisted qingke maturity breeding with optimized plant architecture for promising loci.



中文翻译:

青藏高原青稞大麦(Hordeum vulgare L.)抽穗期性状主要数量性状位点的基因分型测序定位

影响青科成熟度的一个关键特征是抽穗期。抽穗期性状的主要稳定数量性状位点(QTL)的鉴定对于青科成熟度提高具有重要意义。因此,我们对146个品系的F 2群体进行了QTL定位,并构建了具有1,243个重组仓标记的高密度连锁图。在对三个环境中的 F 2:3进行表型分析后,以检测抽穗日期的 QTL。结果表明,鉴定出三个在抽穗日期中起主要作用的 QTL,在两种环境中鉴定出cqHD2H-1cqHD2H-2,并被认为是环境稳定的。该cqHD2H-2,反映了一个稳定的早期抽穗基因座,解释了 27.50% 的表型变异。所述的映射cqHD2H-2在选自F极其标题个体随后进行5组的人群中,cqHD2H-2进一步限定到大约84 MB上2H的区域,并且HORVU2Hr1G087460基因被确定为稻的同源物DTH8通过基因候选区域的 BLASTN 分析。这些结果拓宽了我们对抽穗期遗传基础的理解,并为精细定位和标记辅助青科成熟育种提供线索,优化植物结构以寻找有希望的基因座。

更新日期:2021-09-23
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