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Bacteria-Targeted MRI Probe-Based Imaging Bacterial Infection and Monitoring Antimicrobial Therapy In Vivo
Small ( IF 13.0 ) Pub Date : 2021-09-23 , DOI: 10.1002/smll.202103627 Linyao Li 1 , Peilin Gu 1 , Mengqi Hao 1 , Xiaoli Xiang 1 , Yuting Feng 1 , Xiaokang Zhu 1 , Yang Song 2 , Erqun Song 1
Small ( IF 13.0 ) Pub Date : 2021-09-23 , DOI: 10.1002/smll.202103627 Linyao Li 1 , Peilin Gu 1 , Mengqi Hao 1 , Xiaoli Xiang 1 , Yuting Feng 1 , Xiaokang Zhu 1 , Yang Song 2 , Erqun Song 1
Affiliation
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Despite the significant advances of imaging techniques nowadays, accurate diagnosis of bacterial infections and real-time monitoring the efficacy of antibiotic therapy in vivo still remain huge challenges. Herein, a self-assembling peptide (FFYEGK) and vancomycin (Van) antibiotic molecule co-modified gadolinium (Gd) MRI nanoaggregate probe (GFV) for detecting Staphylococcus aureus (S. aureus) infection in vivo and monitoring the treatment of S. aureus-infected myositis by using daptomycin (Dap) antibiotic as model are designed and fabricated. The as-prepared GFV probe bears Van molecules, making itself good bacteria-specific targeting, and the peptide in the probe can enhance the longitudinal relaxivity rate (r1) after self-assembly due to the π–π stacking. The study showed that, based on the GFV probe, bacterial infections and sterile inflammation can be discriminated, and as few as 105 cfu S. aureus can be detected in vivo with high specificity and accurately. Moreover, the T1 signal of GFV probe at the S. aureus-infected site in mice correlates with the increasing time of Dap treating, indicating the possibility of monitoring the efficacy of antibacterial agents for infected mice based on the as proposed GFV probe. This study shows the potential of GFV probe for diagnosis, evaluation, and prognosis of infectious diseases in clinics.
中文翻译：
基于细菌靶向 MRI 探针的成像细菌感染和体内抗生素治疗监测
尽管如今成像技术取得了重大进展，但细菌感染的准确诊断和体内抗生素治疗的实时监测仍然是巨大的挑战。在此，自组装肽（FFYEGK）和万古霉素（Van）抗生素分子共修饰钆（Gd）MRI纳米聚集探针（GFV）用于检测体内金黄色葡萄球菌（S.aureus）感染和监测金黄色葡萄球菌的治疗-设计并制作了以达托霉素 ( Dap ) 抗生素为模型的感染性肌炎。准备好的 GFV 探针带有Van分子，使其自身具有良好的细菌特异性靶向，并且探针中的肽由于 π-π 堆积而在自组装后可以提高纵向弛豫率 ( r 1 )。研究表明，基于GFV探针，可以区分细菌感染和无菌性炎症，在体内可以高特异性、准确地检测出低至10 5 cfu的金黄色葡萄球菌。此外，GFV 探针在小鼠金黄色葡萄球菌感染部位的 T 1信号与Dap的增加时间相关治疗，表明基于所提出的 GFV 探针监测抗菌剂对受感染小鼠的功效的可能性。该研究显示了 GFV 探针在临床传染病诊断、评估和预后方面的潜力。
更新日期：2021-11-04
中文翻译：
基于细菌靶向 MRI 探针的成像细菌感染和体内抗生素治疗监测
尽管如今成像技术取得了重大进展，但细菌感染的准确诊断和体内抗生素治疗的实时监测仍然是巨大的挑战。在此，自组装肽（FFYEGK）和万古霉素（Van）抗生素分子共修饰钆（Gd）MRI纳米聚集探针（GFV）用于检测体内金黄色葡萄球菌（S.aureus）感染和监测金黄色葡萄球菌的治疗-设计并制作了以达托霉素 ( Dap ) 抗生素为模型的感染性肌炎。准备好的 GFV 探针带有Van分子，使其自身具有良好的细菌特异性靶向，并且探针中的肽由于 π-π 堆积而在自组装后可以提高纵向弛豫率 ( r 1 )。研究表明，基于GFV探针，可以区分细菌感染和无菌性炎症，在体内可以高特异性、准确地检测出低至10 5 cfu的金黄色葡萄球菌。此外，GFV 探针在小鼠金黄色葡萄球菌感染部位的 T 1信号与Dap的增加时间相关治疗，表明基于所提出的 GFV 探针监测抗菌剂对受感染小鼠的功效的可能性。该研究显示了 GFV 探针在临床传染病诊断、评估和预后方面的潜力。
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