Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency < 1%), hyPE2 enables prime editing with efficiencies ranging from 1.1% to 2.9% at up to 34% of target sequences, potentially facilitating prime editing applications.

虽然prime editor 是一种很有前途的基因组编辑方法，但是prime editor 2（PE2）的效率往往不足。在这里，我们通过添加 Rad51 DNA 结合域来生成更有效的 PE2 变体，称为 hyPE2。当在内源性位点进行测试时，hyPE2 显示出比 PE2 高 1.5 或 1.4 倍（范围，0.99 至 2.6 倍）的效率中位数；此外，在 PE2 诱导的引物编辑效率非常低的位点（效率 < 1%），hyPE2 可以在高达 34% 的目标序列上以 1.1% 到 2.9% 的效率进行引物编辑，从而有可能促进引物编辑应用。