当前位置: X-MOL 学术Anal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Versatile Luminol/Dissolved Oxygen/Fe@Fe2O3 Nanowire Ternary Electrochemiluminescence System Combined with Highly Efficient Strand Displacement Amplification for Ultrasensitive microRNA Detection
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-09-23 , DOI: 10.1021/acs.analchem.1c03102
Jia-Li Liu 1 , Rong Yang 1 , Ya-Qin Chai 1 , Ruo Yuan 1
Affiliation  

Herein, a versatile ECL biosensor was fabricated for ultrasensitive detection of microRNA-21 (miRNA-21) from cancer cells based on a novel H2O2-free electrochemiluminescence (ECL) system (luminol/dissolved oxygen/Fe@Fe2O3 nanowires). Compared with the previously reported coreaction accelerator that needed a negative potential to produce reactive oxygen species (ROS), these newly discovered Fe@Fe2O3 nanowires could generate ROS in the detection solution immediately without the application of voltage, which narrowed down the detection potential range to avoid side reactions, favoring their practical application in biological systems. Especially, the Fe@Fe2O3 nanowires could produce H for activating dissolved oxygen into ROS to improve the ECL intensity dramatically, which initiates a novel pathway to promote the generation of ROS for the ECL system. In addition, an original strand displacement amplification coupled with strand displacement reaction (SDA-SDR) was developed to improve the conversion efficiency of the target for sensitive detection of miRNA-21. By virtue of the SDR, a quadruple quenching effect was achieved through each output DNA strand of SDA; hence, the nucleic acid signal amplification efficiency was effectively enhanced. As expected, on account of the superb activation performance of Fe@Fe2O3 nanowires and the outstanding amplification efficiency of the SDR-SDA strategy, the fabricated ECL biosensor realized ultrasensitive detection of miRNA-21 with a detection limit down to 52.5 aM. The established ECL sensing platform ushered a new route for H2O2-free detection and a promising biomarker assay method for clinical diagnosis.

中文翻译:

多功能鲁米诺/溶解氧/Fe@Fe2O3 纳米线三元电化学发光系统结合高效链置换放大用于超灵敏 microRNA 检测

在此,基于新型无 H 2 O 2电化学发光(ECL)系统(鲁米诺/溶解氧/Fe@Fe 2 O 3纳米线)。与之前报道的需要负电位产生活性氧(ROS)的共作用加速器相比,这些新发现的Fe@Fe 2 O 3纳米线无需施加电压即可在检测溶液中立即产生ROS,从而缩小了检测范围避免副反应的潜在范围,有利于它们在生物系统中的实际应用。尤其是 Fe@Fe 2 O3纳米线可产生H 以激活溶解氧为ROS,从而显着提高ECL 强度,这为促进ECL 系统产生ROS 开辟了新途径。此外,还开发了原始链置换扩增与链置换反应(SDA-SDR)相结合的方法,以提高目标的转化效率,用于灵敏检测 miRNA-21。凭借SDR,通过SDA的每条输出DNA链实现了四重猝灭效果;从而有效提高了核酸信号放大效率。正如预期的那样,由于 Fe@Fe 2 O 3出色的活化性能纳米线和 SDR-SDA 策略的出色放大效率,制造的 ECL 生物传感器实现了 miRNA-21 的超灵敏检测,检测限低至 52.5 aM。建立的ECL传感平台为无H 2 O 2检测开辟了一条新途径,并为临床诊断提供了一种有前景的生物标志物检测方法。
更新日期:2021-10-06
down
wechat
bug