Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Characterization of Four Piper Essential Oils (GC/MS and ATR-IR) Coupled to Chemometrics and Their anti-Helicobacter pylori Activity
ACS Omega ( IF 3.7 ) Pub Date : 2021-09-22 , DOI: 10.1021/acsomega.1c03777 Eman Al-Sayed 1 , Haidy A Gad 1 , Dina M El-Kersh 2
ACS Omega ( IF 3.7 ) Pub Date : 2021-09-22 , DOI: 10.1021/acsomega.1c03777 Eman Al-Sayed 1 , Haidy A Gad 1 , Dina M El-Kersh 2
Affiliation
|
Background: Essential oils represent a major class of natural products which are known for their antimicrobial activity. This study aimed to determine the composition of four Piper essential oils by gas chromatography mass spectrometry, attenuated total reflection infrared, and chemometric analysis. Results: Monoterpene was the most predominant class in Piper nigrum and white pepper (87.6 and 80%, respectively) with the dominance of α-pinene, β-pinene, 3-carene, limonene, and β-caryophyllene. Sesquiterpenes represented 50, 19.6, and 12.3% of the essential oils of Piper longum, white pepper, and P. nigrum, respectively. Unlike other species, Piper cubeba oil was found to be rich in aromatics (59%), with eugenol (10.7%) and methyl eugenol (47.4%) representing the major components along with β-myrcene (21.2%) and 1,8-cineole (6.4%). Only P. longum essential oil comprised about 18.2% of alkanes and 13.6% of alkenes. Application of chemometric analysis utilizing GC/MS and ATR-IR data displayed the same segregation pattern where both principal component analysis and hierarchal cluster analysis revealed that white pepper was most closely related to P. nigrum while being completely discriminated from other Piper species. The Piper oils showed promising inhibitory effects on Helicobacter pylori. P. longum oil recorded the most efficient anti-Helicobacter activity [minimum inhibitory concentration (MIC) value of 1.95 μg/ml, which is the same as the MIC of clarithromycin], followed by the oil of white pepper (MIC = 3.90 μg/ml), while P. cubeba and P. nigrum produced the lowest activity (MIC value of 7.81 μg/ml). Conclusion:Piper essential oils can be used as nutritional supplements or therapeutic drugs to protect against H. pylori infection.
中文翻译：
结合化学计量学的四种胡椒精油（GC/MS 和 ATR-IR）的表征及其抗幽门螺杆菌活性
背景: 精油是一大类天然产品，以其抗菌活性而闻名。本研究旨在通过气相色谱质谱、衰减全反射红外和化学计量分析确定四种胡椒精油的成分。结果：单萜是最主要类胡椒和白胡椒（分别为87.6和80％）与α蒎烯，β蒎烯，3-蒈烯，柠檬烯，和β-石竹烯的主导地位。倍半萜表示50，19.6，和的精油12.3％荜茇，白胡椒，和P.葵，分别。与其他物种不同，Piper cubeba发现油中富含芳烃 (59%)，丁香酚 (10.7%) 和甲基丁香酚 (47.4%) 是主要成分，还有 β-月桂烯 (21.2%) 和 1,8-桉树脑 (6.4%)。只有P. longum精油包含约 18.2% 的烷烃和 13.6% 的烯烃。利用 GC/MS 和 ATR-IR 数据的化学计量分析的应用显示了相同的分离模式，其中主成分分析和层次聚类分析表明，白胡椒与P. nigrum 的关系最密切，而与其他胡椒属植物完全区分开来。该派珀油显示出期望的抑制作用幽门螺杆菌。长白菜油记录了最有效的抗螺杆菌活性[最低抑菌浓度 (MIC) 值为 1.95 μg/ml，与克拉霉素的 MIC 相同]，其次是白胡椒油（MIC = 3.90 μg/ml），而P. cubeba和P. nigrum产生的活性最低（MIC 值为 7.81 μg/ml）。结论：胡椒精油可作为营养补充剂或治疗药物来预防幽门螺杆菌感染。
更新日期：2021-10-06
中文翻译：
结合化学计量学的四种胡椒精油（GC/MS 和 ATR-IR）的表征及其抗幽门螺杆菌活性
背景: 精油是一大类天然产品，以其抗菌活性而闻名。本研究旨在通过气相色谱质谱、衰减全反射红外和化学计量分析确定四种胡椒精油的成分。结果：单萜是最主要类胡椒和白胡椒（分别为87.6和80％）与α蒎烯，β蒎烯，3-蒈烯，柠檬烯，和β-石竹烯的主导地位。倍半萜表示50，19.6，和的精油12.3％荜茇，白胡椒，和P.葵，分别。与其他物种不同，Piper cubeba发现油中富含芳烃 (59%)，丁香酚 (10.7%) 和甲基丁香酚 (47.4%) 是主要成分，还有 β-月桂烯 (21.2%) 和 1,8-桉树脑 (6.4%)。只有P. longum精油包含约 18.2% 的烷烃和 13.6% 的烯烃。利用 GC/MS 和 ATR-IR 数据的化学计量分析的应用显示了相同的分离模式，其中主成分分析和层次聚类分析表明，白胡椒与P. nigrum 的关系最密切，而与其他胡椒属植物完全区分开来。该派珀油显示出期望的抑制作用幽门螺杆菌。长白菜油记录了最有效的抗螺杆菌活性[最低抑菌浓度 (MIC) 值为 1.95 μg/ml，与克拉霉素的 MIC 相同]，其次是白胡椒油（MIC = 3.90 μg/ml），而P. cubeba和P. nigrum产生的活性最低（MIC 值为 7.81 μg/ml）。结论：胡椒精油可作为营养补充剂或治疗药物来预防幽门螺杆菌感染。
京公网安备 11010802027423号