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Translocation of silica nanospheres through giant unilamellar vesicles (GUVs) induced by a high frequency electromagnetic field
RSC Advances ( IF 3.9 ) Pub Date : 2021-09-23 , DOI: 10.1039/d1ra05459g Palalle G Tharushi Perera 1, 2 , Nevena Todorova 3 , Zoltan Vilagosh 2 , Olha Bazaka 1 , The Hong Phong Nguyen 4 , Kateryna Bazaka 5 , Russell J Crawford 1 , Rodney J Croft 6 , Irene Yarovsky 3 , Elena P Ivanova 1
RSC Advances ( IF 3.9 ) Pub Date : 2021-09-23 , DOI: 10.1039/d1ra05459g Palalle G Tharushi Perera 1, 2 , Nevena Todorova 3 , Zoltan Vilagosh 2 , Olha Bazaka 1 , The Hong Phong Nguyen 4 , Kateryna Bazaka 5 , Russell J Crawford 1 , Rodney J Croft 6 , Irene Yarovsky 3 , Elena P Ivanova 1
Affiliation
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Membrane model systems capable of mimicking live cell membranes were used for the first time in studying the effects arising from electromagnetic fields (EMFs) of 18 GHz where membrane permeability was observed following exposure. A present lack of understanding of the mechanisms that drive such a rapid change in membrane permeabilization as well as any structural or dynamic changes imparted on biomolecules affected by high-frequency electromagnetic irradiation limits the use of 18 GHz EMFs in biomedical applications. A phospholipid, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) labelled with a fluorescent marker 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (rhodamine-DOPE) was used in constructing the giant unilamellar vesicles (GUVs). After three cycles of exposure, enhanced membrane permeability was observed by the internalisation of hydrophilic silica nanospheres of 23.5 nm and their clusters. All-atom molecular dynamics simulations of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes exposed to high frequency electric fields of different field strengths showed that within the simulation timeframe only extremely high strength fields were able to cause an increase in the interfacial water dynamics characterized by water dipole realignments. However, a lower strength, high frequency EMF induced changes of the water hydrogen bond network, which may contribute to the mechanisms that facilitate membrane permeabilization in a longer timeframe.
中文翻译：
高频电磁场诱导的二氧化硅纳米球通过巨型单层囊泡（GUV）的易位
首次使用能够模拟活细胞膜的膜模型系统来研究 18 GHz 电磁场 (EMF) 产生的影响，在暴露后观察到膜的渗透性。目前对驱动膜透化作用如此快速变化的机制以及受高频电磁辐射影响的生物分子所产生的任何结构或动态变化缺乏了解，限制了 18 GHz EMF 在生物医学应用中的使用。磷脂，1,2-二油酰-sn-甘油-3-磷酸胆碱 (DOPC)，用荧光标记物 1,2-二油酰-sn-甘油-3-磷酸乙醇胺-N- (丽丝胺罗丹明 B 磺酰基) (罗丹明-DOPE) 标记）用于构建巨型单层囊泡（GUV）。经过三个暴露周期后，通过 23.5 nm 亲水性二氧化硅纳米球及其簇的内化，观察到膜渗透性增强。对暴露于不同场强的高频电场的 1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱 (POPC) 膜进行的全原子分子动力学模拟表明，在模拟时间内，只有极高强度的场才能够导致以水偶极重新排列为特征的界面水动力学的增加。然而，较低强度、高频的 EMF 会引起水氢键网络的变化，这可能有助于在较长时间内促进膜透化的机制。
更新日期：2021-09-23
中文翻译：
高频电磁场诱导的二氧化硅纳米球通过巨型单层囊泡（GUV）的易位
首次使用能够模拟活细胞膜的膜模型系统来研究 18 GHz 电磁场 (EMF) 产生的影响，在暴露后观察到膜的渗透性。目前对驱动膜透化作用如此快速变化的机制以及受高频电磁辐射影响的生物分子所产生的任何结构或动态变化缺乏了解，限制了 18 GHz EMF 在生物医学应用中的使用。磷脂，1,2-二油酰-sn-甘油-3-磷酸胆碱 (DOPC)，用荧光标记物 1,2-二油酰-sn-甘油-3-磷酸乙醇胺-N- (丽丝胺罗丹明 B 磺酰基) (罗丹明-DOPE) 标记）用于构建巨型单层囊泡（GUV）。经过三个暴露周期后，通过 23.5 nm 亲水性二氧化硅纳米球及其簇的内化，观察到膜渗透性增强。对暴露于不同场强的高频电场的 1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱 (POPC) 膜进行的全原子分子动力学模拟表明，在模拟时间内，只有极高强度的场才能够导致以水偶极重新排列为特征的界面水动力学的增加。然而，较低强度、高频的 EMF 会引起水氢键网络的变化，这可能有助于在较长时间内促进膜透化的机制。
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