Here we describe a new integrative approach for accurate annotation and quantification of circRNAs named Short Read circRNA Pipeline (SRCP). Our strategy involves two steps: annotation of validated circRNAs followed by a quantification step. We show that SRCP is more sensitive than other individual pipelines and allows for more comprehensive quantification of a larger number of differentially expressed circRNAs. To facilitate the use of SRCP, we generate a comprehensive collection of validated circRNAs in five different organisms, including humans. We then utilize our approach and identify a subset of circRNAs bound to the miRNA-effector protein AGO2 in human brain samples.

中文翻译：

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SRCP：用于精确注释和定量 circRNA 的综合管道

在这里，我们描述了一种新的 circRNA 精确注释和定量综合方法，称为短读 circRNA Pipeline (SRCP)。我们的策略包括两个步骤：对经过验证的 circRNA 进行注释，然后进行量化步骤。我们证明 SRCP 比其他单独的流程更敏感，并且允许对大量差异表达的 circRNA 进行更全面的定量。为了促进 SRCP 的使用，我们在包括人类在内的五种不同生物体中生成了经过验证的 circRNA 的全面集合。然后，我们利用我们的方法并鉴定了人脑样本中与 miRNA 效应蛋白 AGO2 结合的 circRNA 子集。