In this paper, we demonstrate a selective multisite splitting and merging system to perform the type of permutations of reactions required for high-throughput screening. Our approach uses multiple single-layer microfluidic valves as they offer simplicity, biocompatibility, and high-speed operation. These valves are used both to control the generation of droplets and the location of splitting. In the former, the valves are used to completely block some microchannels; in the latter, they create a more modest local geometry variation. The generated droplets act as a vessel that transports different samples around the chip; these vessels can then be subdivided at splitting locations, just as a pipette can be used to dispense a fraction of its total volume. The split droplets enter a merging channel, in which daughter droplets of different types can be merged. We demonstrate the merging of different droplet types in different concentrations at four merging locations.

在本文中，我们展示了一种选择性多位点拆分和合并系统，以执行高通量筛选所需的反应类型排列。我们的方法使用多个单层微流体阀，因为它们具有简单性、生物相容性和高速操作。这些阀门用于控制液滴的产生和分裂的位置。前者是用阀门来完全阻断一些微通道；在后者中，它们创造了更适度的局部几何变化。产生的液滴就像一个容器，在芯片周围运送不同的样品；然后可以在拆分位置细分这些容器，就像可以使用移液器分配其总体积的一小部分一样。分裂的液滴进入合并通道，其中可以合并不同类型的子液滴。我们展示了在四个合并位置不同浓度的不同液滴类型的合并。