Bovine alphaherpesvirus-1 (BoHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is an economically important viral pathogen affecting cattle and buffaloes. Serological assays are mostly used for detection of the antibodies, but variation has been detected in the diagnostic performances of the individual assay. In the present study, four commercially available ELISA kits {two indirect ELISA (kits A and B) and two blocking ELISA (kits C and D)} were evaluated for the detection of antibodies against BoHV-1 in Indian cattle and buffaloes (fitness of purpose). The diagnostic sensitivity (dsn) and specificity (dsp) of these kits were determined by three ways; considering virus neutralization test (VNT) as gold standard test, using pre-test information of the samples, and majority of tests. Screening of 200 known negative sera (124 cattle, 76 buffaloes) sourced from IBR free farms revealed gB based ELISA kits are more specific than the indirect ELISA kits. Testing of 125 known positive sera (81 cattle, 44 buffaloes) suggests kit B be most sensitive followed by kit C, A and D. Interestingly, kit D was found to be most sensitive for detection of vaccination-induced BoHV-1 antibodies followed by kit B. Similar trend were also observed in the limit of dilution experiment performed using known infected and vaccinated sera. VNT was found to be the most specific test and its use as the gold standard test revealed all kits to have more than 99 % sensitivity. All the ELISA kits could detect BoHV-1 specific antibodies in the IBR vaccinated calves as early as 11 days post-vaccination. In Kappa statistics, an almost perfect agreement between the ELISA kits was recorded. The overall performance of the kits in serodiagnosis of IBR as determined by the area under curve in ROC analysis was good.

牛 alphaherpesvirus-1 (BoHV-1) 是传染性牛鼻气管炎 (IBR) 的病原体，是影响牛和水牛的经济上重要的病毒病原体。血清学检测主要用于检测抗体，但已检测到个别检测的诊断性能存在差异。在本研究中，评估了四种市售 ELISA 试剂盒{两种间接 ELISA（试剂盒 A 和 B）和两种阻断 ELISA（试剂盒 C 和 D）}在印度牛和水牛中检测针对 BoHV-1 的抗体（适应性目的）。这些试剂盒的诊断灵敏度 (dsn) 和特异性 (dsp) 通过三种方式确定；将病毒中和测试（VNT）作为金标准测试，使用样本的预测试信息，以及大多数测试。对来自无 IBR 农场的 200 份已知阴性血清（124 头牛，76 头水牛）的筛选表明，基于 gB 的 ELISA 试剂盒比间接 ELISA 试剂盒更具特异性。对 125 份已知阳性血清（81 头牛，44 头水牛）的测试表明，试剂盒 B 最敏感，其次是试剂盒 C、A 和 D。有趣的是，发现试剂盒 D 对疫苗接种诱导的 BoHV-1 抗体的检测最敏感，其次是试剂盒 B。在使用已知感染和接种血清进行的稀释极限实验中也观察到类似的趋势。VNT 被发现是最具体的测试，它作为金标准测试的使用表明所有套件都具有超过 99% 的灵敏度。早在接种后 11 天，所有 ELISA 试剂盒都可以在接种 IBR 的小牛中检测到 BoHV-1 特异性抗体。在 Kappa 统计中，记录了 ELISA 试剂盒之间几乎完美的一致性。根据 ROC 分析中的曲线下面积，试剂盒在 IBR 血清学诊断中的整体性能良好。