Targeted drug delivery is an advanced method that increases the concentration of drug in the specific targeted area in the body. It improves efficacy of treatment and reduces the side effects in drug administration. The use of nanoparticles enhances the bioavailability, in vivo stability, intestinal absorption, solubility, sustained and targeted delivery. Quercetin (Q) is an anticancer agent used in cancer models due to its antioxidant and antitumor properties. Folic acid (FA) is the ligand used to activate receptor mediated endocytosis for targeted delivery of Quercetin. Polydopamine (PD) is pH sensitive and also inhibits angiogenesis. The quinones of PD serves as anchoring points for FA conjugation. In this work, honeycomb structured Nickel oxide (NiO) nanoparticles loaded with quercetin, surface modified with FA and PD was synthesized to target triple negative breast cancer cells. NiO was characterized by XRD, FTIR, Raman Spectroscopy, BET analysis and Zeta Potential. The honeycomb structure was confirmed by SEM. NiO size and morphology was analyzed by TEM. The porous structure of NiO enabled the efficient loading of Quercetin. Hemolysis analysis showed NiO has good hemocompatibility. The drug release profile confirmed a pH sensitive and controlled delivery of Quercetin. The drug release profile expressed higher release at lower pH. The drug release kinetic profile unveils the primary release mechanism to be diffusion controlled. MTT assay were performed against Vero cell line and MDA-MB-231 breast cancer cell line. The nanoformulation showed reduced cytotoxicity and good biocompatibility on Vero cells and appreciable anti-cancer activity on MDA-MB-231 breast cancer cell line.

靶向给药是一种先进的方法，可以增加体内特定靶向区域的药物浓度。它提高了治疗效果并减少了给药中的副作用。纳米颗粒的使用提高了生物利用度、体内稳定性、肠道吸收、溶解性、持续和靶向递送。由于其抗氧化和抗肿瘤特性，槲皮素 (Q) 是一种用于癌症模型的抗癌剂。叶酸 (FA) 是用于激活受体介导的内吞作用以靶向递送槲皮素的配体。聚多巴胺 (PD) 对 pH 值敏感，也抑制血管生成。PD 的醌类作为 FA 结合的锚定点。在这项工作中，蜂窝结构的氧化镍 (NiO) 纳米颗粒负载槲皮素，合成了用 FA 和 PD 修饰的表面以靶向三阴性乳腺癌细胞。NiO 通过 XRD、FTIR、拉曼光谱、BET 分析和 Zeta 电位表征。通过SEM确认蜂窝结构。NiO 的尺寸和形态通过 TEM 分析。NiO 的多孔结构使槲皮素的有效负载成为可能。溶血分析表明NiO具有良好的血液相容性。药物释放曲线证实了槲皮素的 pH 敏感和受控递送。药物释放曲线表示在较低 pH 下释放较高。药物释放动力学曲线揭示了受扩散控制的主要释放机制。针对 Vero 细胞系和 MDA-MB-231 乳腺癌细胞系进行 MTT 测定。