Microorganisms have been contemplated as a promising source for the inexhaustible synthesis of many novel materials utilizing renewable sources. Among many of such products, polyhydroxyalkanoate (PHA) remains as an essential biodegradable polymer with functions similar to conventional plastics. Bacillus endophyticus is capable of accumulating biopolymer PHA in nutrient limiting conditions with excess of carbon source. Screening and optimizing the parameters for increased PHA production was done statistically. The optimized medium gave a maximum yield of 46.57% which was in well agreement with the given predicted value provided by response surface methodology model yield of 47.02%. Optimal media conditions when extrapolated in bioreactor gave an even higher production percentage of 49.9. This is the first report highlighting 49% of polyhydroxybutyrate statistically using sucrose as a source. The main highlight of the study was the use of wild type strain for producing high quality PHA using simple carbon source which can be a starting platform for using this strain for large scale PHA production industrially. FTIR and ¹HNMR analysis confirmed the polymer produced.

微生物被认为是利用可再生资源不断合成许多新材料的有希望的来源。在许多此类产品中，聚羟基链烷酸酯 (PHA) 仍然是一种重要的可生物降解聚合物，其功能类似于传统塑料。内生芽孢杆菌能够在碳源过剩的营养限制条件下积累生物聚合物 PHA。统计筛选和优化增加PHA产量的参数。优化后的培养基的最大产率为 46.57%，这与响应面法模型产率 47.02% 提供的给定预测值非常吻合。当在生物反应器中外推时，最佳培养基条件给出了 49.9 的更高生产百分比。这是第一份在统计上强调 49% 的聚羟基丁酸酯使用蔗糖作为来源的报告。该研究的主要亮点是使用野生型菌株使用简单的碳源生产高质量的 PHA，这可以作为将该菌株用于工业大规模 PHA 生产的起始平台。FTIR 和¹HNMR分析证实了产生的聚合物。