AIEgen-Based Lifetime-Probes for Precise Furin Quantification and Identification of Cell Subtypes,Advanced Materials

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AIEgen-Based Lifetime-Probes for Precise Furin Quantification and Identification of Cell Subtypes
Advanced Materials ( IF 27.4 ) Pub Date : 2021-09-23 , DOI: 10.1002/adma.202104615
Hui Jia ₁ , Defang Ding ₁ , Jingjing Hu ₁ , Jun Dai ₂ , Juliang Yang ₁ , Guogang Li ₁ , Xiaoding Lou ₁ , Fan Xia ₁

Affiliation

Biochemical sensing probes based on aggregation-induced-emission luminogens (AIEgens) are widely used in biological imaging and therapy, chemical sensing, and material sciences. However, it is still a great challenge to quantify the targets through fluorescence intensity of AIEgen probes due to their undesirable aggregations. Here, a PyTPA-ZGO probe with three lifetime signals for precise quantification of furin is constructed: the lifetime signal 1 and signal 2 comes from AIEgen PyTPA-P (τ_Pn) and inorganic nanoparticles Zn₂GeO₄:Mn²⁺-NH₂ (τ_Zn), respectively, while the lifetime signal 3 is marked as the composite dual-lifetime signal (CDLS_n,

C D L S_{n} = \frac{τ_{Z n}}{τ_{P n}}

). In contrast, the fluorescence intensity signal of PyTPA-P shows defectively quantitative performance. Furthermore, it is found that the CDLS_n exhibits higher significant differences than the two other lifetime signals (τ_Pn and τ_Zn) thanks to its wide range between the maximum and minimum signal values and small standard deviation. Therefore, CDLS_n is further used to accurately identify cell subtypes based on the specific concentration of furin in each subtype. The lifetime criterion can realize precise quantification, and it should be a promising direction of AIEgen-based quantitative analysis in the future.

中文翻译：

用于精确弗林蛋白酶定量和细胞亚型鉴定的基于 AIEgen 的寿命探针

基于聚集诱导发射发光体 (AIEgens) 的生化传感探针广泛用于生物成像和治疗、化学传感和材料科学。然而，由于 AIEgen 探针的不受欢迎的聚集，通过 AIEgen 探针的荧光强度来量化目标仍然是一个巨大的挑战。在这里，构建了具有三个寿命信号的 PyTPA-ZGO 探针，用于精确定量弗林蛋白酶：寿命信号 1 和信号 2 来自 AIEgen PyTPA-P (τ _Pn ) 和无机纳米粒子 Zn ₂ GeO ₄ :Mn ²⁺ -NH ₂ (τ _Zn ), 而寿命信号 3 被标记为复合双寿命信号 (CDLS _n ,

C D 大号 {小号}_{n} = \frac{τ_{Z n}}{τ_{磷 n}}

）。相反，PyTPA-P 的荧光强度信号显示出有缺陷的定量性能。此外，发现 CDLS _n表现出比其他两个寿命信号（τ _Pn和 τ _Zn）更大的显着差异，这是因为它在最大和最小信号值之间的范围很广，并且标准偏差很小。因此，CDLS _n进一步用于根据每个亚型中弗林蛋白酶的特定浓度来准确识别细胞亚型。寿命准则可以实现精确量化，应该是未来基于AIEgen的量化分析的一个有前途的方向。

更新日期：2021-11-09

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