In this study, a green solvent (water: ethanol; 98:2) was applied to extract β-carotene (BC) from carrot pomace powder (CP). The low solubility (<5%) of BC was improved (~21%) by enzymatic hydrolysis of CP with a commercial pectinase (61 U/mL, 60 min); enzymatic liquefaction of CP improved the extraction efficiency up to 90%. Also, the nanoemulsions (NEs) based on different surfactants (Lecithin; Lec, Tween 80; T80, Tween 20; T20, and Span 20; S20) enhanced the extraction efficiency of BC. Up to >91%. HPLC data revealed that BC (60%) and α-carotene (29%) were the major carotenoids extracted into micelles of NEs, whereas lutein (9%) and lycopene (2%) as the minor ones. Finally, stability monitoring confirmed that BC in ethanolic extract from CP was completely lost, while BC in NE platform showed good stability of ~25% and 32% as exposed to UV-light and acidic conditions (pH ~ 4), respectively.

在本研究中，采用绿色溶剂（水：乙醇；98：2）从胡萝卜渣粉 (CP) 中提取 β-胡萝卜素 (BC)。通过使用商业果胶酶（61 U/mL，60 分钟）对 CP 进行酶促水解，BC 的低溶解度（<5%）得到改善（~21%）；CP的酶促液化将提取效率提高到90%。此外，基于不同表面活性剂（卵磷脂；Lec，吐温 80；T80，吐温 20；T20 和 Span 20；S20）的纳米乳液 (NE) 提高了 BC 的提取效率。高达 >91%。HPLC 数据显示 BC (60%) 和 α-胡萝卜素 (29%) 是提取到 NEs 胶束中的主要类胡萝卜素，而叶黄素 (9%) 和番茄红素 (2%) 是次要的。最后，稳定性监测证实 CP 乙醇提取物中的 BC 完全丢失，