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Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota
FEMS Microbiology Letters ( IF 2.2 ) Pub Date : 2021-09-21 , DOI: 10.1093/femsle/fnab128
Cheikh Ibrahima Lo 1, 2 , Niokhor Dione 2, 3 , Aminata Mbaye 2, 3 , Patricia Fernández-Mellado Gómez 2, 3 , Issa Isaac Ngom 2, 3 , Camille Valles 2, 3 , Stéphane Alibar 2, 3 , Jean-Christophe Lagier 2, 3 , Florence Fenollar 1, 2 , Pierre-Edouard Fournier 1, 2 , Didier Raoult 2, 3 , Seydina M Diene 2, 3
Affiliation  

Strain Marseille-P3519T isolated from the fecal flora of a 25-year-old healthy French woman was a Gram-positive anaerobic bacterium, non-motile and non-spore forming. The 16S rRNA gene sequence of Marseille-P3519 showed 97.73% of sequence similarity with Limosilactobacillus reuteri DSM 20016, the closest species, phylogenetically. Furthermore, the average nucleotide identity of strain Marseille-3519 with its closest related species was 75.8% that was very below the recommended threshold (>95–96%). Its genome had 2 237 367 bp with 45.42 mol% of G + C content. Major fatty acids were C16:0 (50.8%), C18:1n9 (18.0%), C18:2n6 (9.8%) and C19:1n9 (8.9%). It was catalase negative and fermented glycerol, glucose, fructose, D-maltose, lactose and mannose. These findings support that strain Marseille-P3519 ( = CSURP3519 = CECT 30110) is a new member of the genus Limosilactobacillus for which the name Limosilactobacillus caccae sp. nov., is proposed.

中文翻译:

Limosilactobacillus caccae sp。十一月,从人类肠道微生物群中分离出的一种新细菌

从一名 25 岁健康法国妇女的粪便菌群中分离出的菌株 Marseille-P3519 T是一种革兰氏阳性厌氧菌,不活动且不形成孢子。Marseille-P3519 的 16S rRNA 基因序列显示与Limosilactobacillus reuteri DSM 20016 在系统发育上最接近的物种的序列相似性为 97.73%。此外,菌株 Marseille-3519 与其最近相关物种的平均核苷酸同一性为 75.8%,远低于推荐阈值 (>95–96%)。其基因组全长2 237 367 bp,G+C含量为45.42 mol%。主要脂肪酸为 C 16:0 (50.8%)、C 18:1n9 (18.0%)、C 18:2n6 (9.8%) 和 C 19:1n9(8.9%)。它是过氧化氢酶阴性发酵甘油、葡萄糖、果糖、D-麦芽糖、乳糖和甘露糖。这些发现支持菌株 Marseille-P3519(= CSURP3519 = CECT 30110)是Limosilactobacillus属的新成员,其名称为Limosilactobacillus caccae sp。十一月,被提议。
更新日期:2021-10-10
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