Combined therapy can improve the curative effect and reduce side effect, so it has a broad application prospect in the treatment of bacterial infections especially bacterial biofilm infections. Herein, we propose a photothermal-activated multifunctional nano-antibacterial platform that is constructed by introducing indocyanine green (ICG) photosensitizers and silver nanoparticles (AgNPs) onto molybdenum disulfide (MoS₂) nanosheets surface. The constructed nanoplatform (MoS₂/ICG/Ag) has the combined chemo/photothermal/photodynamic triple-mode antibacterial capability under a single 808 nm laser irradiation. Briefly, the photonic hyperthermia generated by MoS₂ nanosheets can not only directly kill bacteria, but also accelerate the release of ICG and silver ions, and the latter is a commonly used chemical antibacterial reagent. The released ICG can catalyze the conversion of oxygen into singlet oxygen with the help of 808 nm light, achieving the photodynamic sterilization. Encouragingly, the loaded ICG and AgNPs can in turn enhance the heat, which is a mutually reinforcing effect that can produce a combined or even synergistic therapeutic effect. In vitro experiments demonstrate that the MoS₂/ICG/Ag with triple bactericidal modalities has good broad-spectrum antibacterial performance. Importantly, it can remarkably inhibit the S. aureus biofilms formation and effectively eradicate the bacteria deep in the biofilms. In addition, the MoS₂/ICG/Ag is successfully used for treating S. aureus biofilms-infected wound in vivo with minimal toxicity. Taken together, the developed MoS₂/ICG/Ag nanoplatform may serve as a potential nano-antibacterial agent for photothermally activated multimodal combined antibacterial and antibiofilm therapy.

联合用药可提高疗效，减少副作用，因此在治疗细菌感染尤其是细菌生物膜感染方面具有广阔的应用前景。在此，我们提出了一种光热激活的多功能纳米抗菌平台，该平台是通过将吲哚菁绿 (ICG) 光敏剂和银纳米粒子 (AgNPs) 引入二硫化钼 (MoS ₂ ) 纳米片表面而构建的。构建的纳米平台（MoS ₂ /ICG/Ag）在单次808 nm激光照射下具有组合的化学/光热/光动力三模式抗菌能力。简而言之，MoS ₂产生的光子热疗纳米片不仅可以直接杀灭细菌，还可以加速ICG和银离子的释放，后者是一种常用的化学抗菌试剂。释放的ICG可以在808nm光的帮助下催化氧气转化为单线态氧，实现光动力灭菌。令人鼓舞的是，负载的 ICG 和 AgNPs 可以反过来增强热量，这是一种相辅相成的效果，可以产生组合甚至协同的治疗效果。体外实验表明，具有三重杀菌方式的MoS ₂ /ICG/Ag具有良好的广谱抗菌性能。重要的是，它可以显着抑制金黄色葡萄球菌生物膜形成并有效根除生物膜深处的细菌。此外，MoS ₂ /ICG/Ag 已成功用于在体内治疗金黄色葡萄球菌生物膜感染的伤口，并且毒性最小。总之，开发的MoS ₂ /ICG/Ag纳米平台可作为一种潜在的纳米抗菌剂，用于光热激活多模式联合抗菌和抗生物膜治疗。