One of the most critical challenges in tissue engineering is the fabrication of porous scaffolds with high porosity and proper mechanical properties. Conventional synthetic scaffolds are typically made of ceramic composed with other ceramics; however, a better combination of nanocomposite can be obtained using magnetite nanoparticles (MNPs), which benefits both ceramic materials. The current study used the space holder method and mechanical activation to create a porous magnetic-zinc calcium bio-nanocomposite scaffold for bone tissue applications. The samples were made with varying amounts of reinforcement (0, 5, 10, and 15 wt%). The addition of sodium chloride has the main effect on the porosity size of the architecture. The prepared magnetic-zinc calcium bio-nanocomposite scaffold was coated with a 5% solution of chitosan polymer, and its mechanical and biological properties were investigated. Powders and scaffolds were examined by X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy. The cell viability and non-toxicity of porous scaffolds were evaluated by MTT assay. The results showed that the addition of 15 wt% of MNPs changed the mechanical and biological properties of the composite scaffold. According to the SEM results, addition of 15% MNPs did not significantly change the porosity. However, it improves the porosity percentages. After being placed in the simulated body fluid (SBF), SEM images of the scaffolds showed the formation of a bone-like apatite layer on the surfaces of the sample with a higher amount of MNPs. Biocompatibility assessment by MTT test showed that composite scaffolds did not show any toxicity in contact with bone marrow stem cells and increased cell growth and proliferation. The mechanical simulation shows that the specimen's mechanical strength can be predicted by low error and can be a suitable option for bone tissue engineering applications.

组织工程中最关键的挑战之一是制造具有高孔隙率和适当机械性能的多孔支架。传统的合成支架通常由陶瓷与其他陶瓷组成；然而，使用磁铁矿纳米颗粒 (MNP) 可以获得更好的纳米复合材料组合，这对两种陶瓷材料都有好处。目前的研究使用空间支架方法和机械激活来创建用于骨组织应用的多孔磁性锌钙生物纳米复合支架。样品由不同数量的增强材料（0、5、10 和 15 重量%）制成。氯化钠的添加对结构的孔隙尺寸有主要影响。制备的磁锌钙生物纳米复合支架用5%的壳聚糖聚合物溶液包被，并对其力学和生物学特性进行了研究。通过 X 射线衍射 (XRD)、扫描电子显微镜 (SEM) 和傅里叶变换红外 (FTIR) 光谱检查粉末和支架。通过MTT法评估多孔支架的细胞活力和无毒性。结果表明，添加 15 wt% 的 MNPs 改变了复合支架的力学和生物学性能。根据 SEM 结果，添加 15% MNPs 不会显着改变孔隙率。然而，它改进了孔隙率百分比。放置在模拟体液 (SBF) 中后，支架的 SEM 图像显示在具有较高 MNP 量的样品表面形成骨状磷灰石层。通过 MTT 测试的生物相容性评估表明，复合支架在与骨髓干细胞接触时没有表现出任何毒性，并增加了细胞的生长和增殖。力学模拟表明，标本的机械强度可以通过低误差预测，是骨组织工程应用的合适选择。