Enterovirus A (EV-A) species are the main agents responsible for hand, foot, and mouth disease (HFMD), a serious public health concern. Lack of appropriate reagents prevents the mechanistic study of these virus infections. 2C protein, a non-structural protein of Enterovirus, is crucial for viral replication and antiviral immunity. Here, preparation and testing of a monoclonal antibody by immunizing mice with Coxsackievirus A10 protein 2C (CVA10-2C) was reported. This antibody could identify most EV-A types, both conventional and unconventional groups. We also mapped the antibody epitope SLATGIIARA, which is highly conserved in EV-A species and located in the ATPase domain. Some key amino acids include G140, I141, I142, and R144. In conclusion, we generated a recombinant monoclonal antibody against multiple EVA types and confirmed its performance, which may facilitate the future study of Enterovirus A infection and many potential applications, such as the diagnosis of pathogen or the development of antiviral therapies.

肠道病毒 A (EV-A) 物种是导致严重的公共卫生问题手足口病 (HFMD) 的主要病原体。缺乏合适的试剂阻碍了对这些病毒感染的机制研究。2C 蛋白是肠道病毒的一种非结构蛋白，对病毒复制和抗病毒免疫至关重要。本文报道了通过用柯萨奇病毒 A10 蛋白 2C (CVA10-2C) 免疫小鼠来制备和测试单克隆抗体。这种抗体可以识别大多数 EV-A 类型，包括常规和非常规组。我们还绘制了抗体表位 SLATGIIARA，它在 EV-A 物种中高度保守，位于 ATPase 结构域。一些关键氨基酸包括 G140、I141、I142 和 R144。总之，我们产生了针对多种 EVA 类型的重组单克隆抗体，并确认了其性能，