Rapid identification of high-affinity aptamers against human TNFα: In vitro selection coupled with microbead-assisted capillary electrophoresis (MACE) separation was performed to rapidly identify anti-TNFα aptamers; it was completed in only three rounds. Selected Apt14 showed the strongest inhibition activity according to the cell viability assay. Furthermore, by introducing a complementary linker to the aptamers, facile dimerization was performed, resulting in enhanced affinity and inhibition activity compared to that of the monomer.

中文翻译：

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通过微珠辅助毛细管电泳 (MACE)-SELEX 加速发现有效的生物活性抗 TNFα 适体

快速鉴定抗人TNFα的高亲和力适体：体外选择结合微珠辅助毛细管电泳（MACE）分离，快速鉴定抗TNFα适体；它只用了三轮就完成了。根据细胞活力测定，选定的 Apt14 显示出最强的抑制活性。此外，通过将互补接头引入适配体，进行了简单的二聚化，与单体相比，亲和力和抑制活性增强。