The Chinese mitten crab (Eriocheir sinensis) mainly depends on lipids and carbohydrates to meet the energy requirements for its growth and rapid ovarian development during the exogenous vitellogenesis. Therefore, this study aimed to explore the effects of dietary icariin supplement on the hepatopancreas’ glycolipid metabolism and energy metabolism during this vital stage. Biochemical analysis revealed the apparent differential effects of icariin on the key indicators involving glycolipid metabolism. The functional mechanisms of icariin on glycolipid metabolism were systematically investigated using transcriptomic and metabolomic analysis. After 100 mg/kg icariin supplementation, fifty differential expression genes mainly involving in glycolysis, gluconeogenesis, fatty acid β-oxidation, tricarboxylic acid cycle and mitochondrial oxidative phosphorylation pathway were found. In addition, forty differential metabolites mainly involving the metabolism process of pyruvate metabolism, fatty acid metabolism, arachidonic acid metabolism and steroid hormone synthesis were identified. Besides, the results of RT-PCR and Western blot verified several key transcription factors related to glycolipid and energy metabolism; the results demonstrated that icariin could strengthen glycolipid metabolism and energy metabolism of E. sinensis by activating related genes. This would facilitate the further understanding of the metabolism and regulation mechanism of nutrition and energy during the exogenous vitellogenesis in crustaceans.

中文翻译：

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基于转录组学和代谢组学研究淫羊藿苷对外源性卵黄发育过程中淫羊藿苷对雌性中华绒螯蟹肝胰腺糖脂代谢和能量代谢的调控作用

中华绒螯蟹 ( Eriocheir sinensis)) 主要依靠脂质和碳水化合物来满足其在外源性卵黄生成过程中生长和卵巢快速发育的能量需求。因此，本研究旨在探讨膳食补充淫羊藿苷对肝胰腺这一生命阶段糖脂代谢和能量代谢的影响。生化分析显示淫羊藿苷对涉及糖脂代谢的关键指标有明显的不同影响。使用转录组学和代谢组学分析系统地研究淫羊藿苷对糖脂代谢的功能机制。补充100 mg/kg淫羊藿苷后，发现了50个差异表达基因，主要涉及糖酵解、糖异生、脂肪酸β-氧化、三羧酸循环和线粒体氧化磷酸化途径。此外，鉴定出40种差异代谢物，主要涉及丙酮酸代谢、脂肪酸代谢、花生四烯酸代谢和类固醇激素合成的代谢过程。此外，RT-PCR和Western blot结果验证了与糖脂和能量代谢相关的几个关键转录因子；结果表明，淫羊藿苷可以加强糖脂代谢和能量代谢。E. sinensis通过激活相关基因。这将有助于进一步了解甲壳类动物外源性卵黄生成过程中营养和能量的代谢和调节机制。