In this present study, Rosmarinus officinalis essential oil and its new microemulsions (MEs) were evaluated both for the in vitro antibacterial and antifungal activity. The essential oil composition was verified initially both by GC-FID and GC/MS, where camphor (19.5%), 1,8-cineol (12%), and verbenone (11.1%) were characterized as the main constituents. A titration method was used and utilized to formulate the MEs. The preparation was formulated using isopropyl myristate as an oil phase, where sorbitan monolaurate and macrogol-glycerol ricinoleate were used as surfactants. In the same system, ethanol was utilized as co-surfactant and distilled water as aqueous phase. A total of 0.1% (w/w) of R. officinalis essential oil was dissolved within the formulation, and evaluated for characteristic parameters such as viscosity, pH, electrical conductivity, refractive index, polydispersity index (PDI), droplet size, and zeta potential, respectively. The pH value was 6.2, where the average microemulsion droplet size was 4.58 ± 0.137 nm; the PDI value was found as 0.219 ± 0.016. The viscosity of the microemulsion was determined as 6.93 ± 0.015 cP. The conductivity of MEs was found as 10 μS/cm; finally, the zeta potential was determined as 0.391 ± 0.012 mV. The antimicrobial potential of the preparations was compared using the in vitro broth microdilution, and poisoned PDA assay. A significant increase in the anti-Candidal activity of the micro-emulsified essential oil (15.6 μg/ml) was observed, however, the antibacterial susceptibility was rather low at the tested concentrations. In addition, the novel microemulsion preparation significantly inhibited mycelial growth of the plant pathogens used in the study, suggesting its selective antifungal potential.

中文翻译：

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Rosmarinus officinalis L. 精油封装在新的微乳液配方中，以增强抗菌活性

在本研究中，评估了迷迭香精油及其新型微乳液 (ME) 的体外抗菌和抗真菌活性。精油成分最初通过 GC-FID 和 GC/MS 验证，其中樟脑 (19.5%)、1,8-桉树脑 (12%) 和马鞭草酮 (11.1%) 被表征为主要成分。使用滴定法并用于配制 ME。该制剂使用肉豆蔻酸异丙酯作为油相进行配制，其中脱水山梨糖醇单月桂酸酯和聚乙二醇-甘油蓖麻油酸酯用作表面活性剂。在同一系统中，乙醇用作助表面活性剂，蒸馏水用作水相。总共 0.1% (w/w) 的R. officinalis将精油溶解在配方中，并分别评估粘度、pH、电导率、折射率、多分散性指数 (PDI)、液滴大小和 zeta 电位等特征参数。pH值为6.2，平均微乳液液滴尺寸为4.58±0.137 nm；PDI 值为 0.219 ± 0.016。微乳液的粘度测定为 6.93 ± 0.015 cP。ME的电导率为10 μS/cm；最后，zeta 电位确定为 0.391 ± 0.012 mV。使用体外肉汤微量稀释和中毒 PDA 测定比较了制剂的抗菌潜力。然而，观察到微乳化精油 (15.6 μg/ml) 的抗念珠菌活性显着增加，在测试浓度下，抗菌敏感性相当低。此外，新型微乳液制剂显着抑制了研究中使用的植物病原体的菌丝体生长，表明其具有选择性抗真菌潜力。