Abstract

The aim of this study was to evaluate the anxiolytic-like effect of chrysophanol (CHRY), isolated from hexane extract of Senna cana stem and its possible mechanism of action. CHRY was obtained through chromatographic treatments and its identity was confirmed by uni and bidimensional RMN¹H and RMN¹³C. Adult zebrafish (n = 6/group) were treated (with CHRY (4.0 or 12.0 or 40.0 mg/Kg; 20 µL; intraperitoneally) and submitted to acute toxicity and open field tests. Subsequently, other groups (n = 6/each) received CHRY for the analysis of its effect on the Light & Dark Test. The participation of the GABAergic system was also assessed using the diazepam (GABA_A receptor agonist) and flumazenil (GABA_A receptor antagonist). CHRY was considered non-toxic, it did not reduce the locomotor activity, and showed an anxiolytic-like effect. This effect was reduced by pre-treatment with flumazenil. The results suggest that CHRY is an anxiolytic-like agent mediated via the GABAergic system.

摘要

本研究的目的是评估从番泻叶茎的己烷提取物中分离的大黄酚 (CHRY) 的类抗焦虑作用及其可能的作用机制。CHRY 是通过色谱处理获得的，其身份通过单维和二维 RMN ¹ H 和 RMN ¹³ C 确认。成年斑马鱼（n = 6/组）进行了处理（用 CHRY（4.0 或 12.0 或 40.0 mg/Kg；20 µL；腹腔注射）并进行急性毒性和野外试验。随后，其他组（n = 6/每）接受 CHRY 以分析其对光和暗测试的影响。还使用地西泮评估了 GABAergic 系统的参与（GABA _A受体激动剂）和氟马西尼（GABA _A受体拮抗剂）。CHRY 被认为是无毒的，它不会降低运动活性，并显示出类抗焦虑作用。用氟马西尼预处理降低了这种影响。结果表明，CHRY 是一种通过 GABA 能系统介导的类抗焦虑剂。