The mechanisms generating variability in viruses are diverse. Variability allows baculoviruses to evolve with their host and with changes in their environment. We examined the role of one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution to the variability of the virus under laboratory conditions. A mixture of natural isolates (ChinNPV‑Mex1) contained two genetic variants that dominated over other variants in individual larvae that consumed high (ChinNPV-K) and low (ChinNPV-E) concentrations of inoculum. Studies on the ChinNPV‑K variant indicated that it was capable of generating novel variation in a concentration-dependent manner. In cell culture, cells inoculated with high concentrations of ChinNPV-K produced OBs with the ChinNPV-K REN profile, whereas a high diversity of ChinNPV variants was recovered following plaque purification of low concentrations of ChinNPV-K virion inoculum. Interestingly, the ChinNPV-K variant could not be recovered from plaques derived from low concentration inocula originating from budded virions or occlusion-derived virions of ChinNPV-K. Genome sequencing revealed marked differences between ChinNPV-K and ChinNPV-E, with high variation in the ChinNPV-K genome, mostly due to single nucleotide polymorphisms. We conclude that ChinNPV‑K is an unstable genetic variant that is responsible for generating much of the detected variability in the natural ChinNPV isolates used in this study.

中文翻译：

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Chrysodeixis 变异性的产生包括核多角体病毒 (ChinNPV)：单一变异体的作用

产生病毒变异性的机制是多种多样的。变异性允许杆状病毒随着宿主和环境的变化而进化。我们检查了金黄蜂的一种遗传变异体的作用，包括核多角体病毒 (ChinNPV) 及其在实验室条件下对病毒变异性的贡献。天然分离物 (ChinNPV-Mex1) 的混合物含有两种遗传变异，它们在消耗高 (ChinNPV-K) 和低 (ChinNPV-E) 接种物浓度的个体幼虫中占主导地位。对 ChinNPV‑K 变体的研究表明，它能够以浓度依赖性方式产生新的变异。在细胞培养中，接种高浓度 ChinNPV-K 的细胞产生具有 ChinNPV-K REN 谱的 OB，而在低浓度的 ChinNPV-K 病毒粒子接种物的噬斑纯化后恢复了高度多样性的 ChinNPV 变体。有趣的是，ChinNPV-K 变体无法从源自 ChinNPV-K 出芽病毒体或闭塞病毒体的低浓度接种物的斑块中恢复。基因组测序揭示了 ChinNPV-K 和 ChinNPV-E 之间的显着差异，ChinNPV-K 基因组的变异很大，主要是由于单核苷酸多态性。我们得出结论，ChinNPV‑K 是一种不稳定的遗传变异体，它负责在本研究中使用的天然 ChinNPV 分离株中产生大部分检测到的变异性。ChinNPV-K 变体无法从源自 ChinNPV-K 的出芽病毒体或闭塞病毒体的低浓度接种物的斑块中恢复。基因组测序揭示了 ChinNPV-K 和 ChinNPV-E 之间的显着差异，ChinNPV-K 基因组的变异很大，主要是由于单核苷酸多态性。我们得出结论，ChinNPV‑K 是一种不稳定的遗传变异体，它负责在本研究中使用的天然 ChinNPV 分离株中产生大部分检测到的变异性。ChinNPV-K 变体无法从源自 ChinNPV-K 的出芽病毒体或闭塞病毒体的低浓度接种物的斑块中恢复。基因组测序揭示了 ChinNPV-K 和 ChinNPV-E 之间的显着差异，ChinNPV-K 基因组的变异很大，主要是由于单核苷酸多态性。我们得出结论，ChinNPV‑K 是一种不稳定的遗传变异体，它负责在本研究中使用的天然 ChinNPV 分离株中产生大部分检测到的变异性。