Obesity is a chronic systemic inflammatory disease, which occurs when energy intake exceeds the energy consumption. Therefore, controlling energy intake or increasing physical consumption can effectively control obesity. However, in reality, it is very difficult for the majority of obese patients to lose weight by autonomously controlling diet. In this study, oral shRNA/yeast microcapsules were constructed with non-virus-mediated IL-1β shRNA interference vectors and non-pathogenic Saccharomyces cerevisiae. Moreover, high-fat diet induced obese mice were established to assess the weight loss effect of IL-1β shRNA/yeast microcapsules via the oral route. After IL-1β shRNA/yeast treatment, body weight and fat weight was reduced. Compared with the control group, higher average food intake but lower energy conversion rate was observed in IL-1β shRNA/yeast group. In addition, lipid metabolism related cytokines and blood glucose concentration in the circulating blood was improved after IL-1β shRNA/yeast treatment. Yeast microcapsules mediated IL-1β shRNA delivery can effectively improve obesity. Noteworthy, this kind of non-diet-controlled weight loss strategy does not need diet control, and shows good biocompatibility. It is good news to obese patients who need to lose weight but cannot control their diet.

中文翻译：

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通过非致病性酵母微胶囊介导的 shRNA 递送对 HFD 肥胖症进行口服基因治疗

肥胖是一种慢性全身炎症性疾病，当能量摄入超过能量消耗时就会发生。因此，控制能量摄入或增加体力消耗可以有效控制肥胖。但现实中，大多数肥胖患者通过自主控制饮食来减肥是非常困难的。在本研究中，使用非病毒介导的IL-1β shRNA 干扰载体和非致病性酿酒酵母构建了口服 shRNA/酵母微胶囊。此外，建立了高脂饮食诱导的肥胖小鼠，以评估IL-1β shRNA/酵母微胶囊通过口服途径的减肥效果。经过IL-1βshRNA/酵母处理，体重和脂肪重量减少。与对照组相比，IL-1β shRNA/酵母组的平均食物摄入量更高，但能量转化率更低。此外，IL-1β shRNA/酵母治疗后循环血中脂质代谢相关的细胞因子和血糖浓度得到改善。酵母微胶囊介导的IL-1β shRNA 递送可有效改善肥胖。值得注意的是，这种非节食控制的减肥策略不需要节食控制，表现出良好的生物相容性。对于需要减肥但无法控制饮食的肥胖患者来说，这是个好消息。