Due to their intrinsic nuclease resistance, L-oligonucleotides are being increasingly utilized in the development of molecular tools and sensors. Yet, it remains challenging to synthesize long L-oligonucleotides, potential limiting future applications. Herein, we report straightforward and versitile approach to assemble long L-RNAs from two or more shorter fragments using T4 RNA ligase 1. We show that this approach is compatible with the assembly of several classes of functional L-RNA, which we highlight by generating a 124 nt L-RNA biosensor that functions in serum.

中文翻译：

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通过天然 RNA 连接组装长 L-RNA

由于其固有的核酸酶抗性，L-寡核苷酸正越来越多地用于分子工具和传感器的开发。然而，合成长L-寡核苷酸仍然具有挑战性，这可能会限制未来的应用。在这里，我们报告了使用 T4 RNA 连接酶 1 从两个或多个较短片段组装长L- RNA的直接且多功能的方法。我们表明，这种方法与几类功能性L- RNA的组装兼容，我们通过生成一种在血清中起作用的 124 nt L- RNA 生物传感器。