Hydrogen polysulfide (H₂S_n, n >1), as a direct oxidizing form of hydrogen sulfide, is closely associated with intestinal diseases such as ulcerative colitis (UC). A probe that can selectively detect H₂S_n in the pathological environment of UC is in urgent demand. Ratiometric probes are powerful in the quantitative detection of H₂S_n in living organisms. Herein, we developed a ratiometric fluorescent probe TCFPB–H₂S_n for selective detection of H₂S_n in vitro and in vivo. Significantly, TCFPB–H₂S_n demonstrated a fast, sensitive and specific detection performance for H₂S_n, and has excellent lysosomal targeting ability and aggregation-induced emission (AIE) characteristics. More importantly, TCFPB–H₂S_n was the first probe to achieve endogenous H₂S_n imaging in acute ulcerative colitis successfully.

中文翻译：

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一种易于获得的溶酶体靶向比率荧光探针，具有聚集诱导发射特性，用于急性溃疡性结肠炎中的多硫化氢可视化

多硫化氢（H ₂ S _n，n >1）作为硫化氢的直接氧化形式，与溃疡性结肠炎（UC）等肠道疾病密切相关。迫切需要一种能够选择性检测UC病理环境中H ₂ S _n的探针。比率探针在活生物体中 H ₂ S _n的定量检测方面非常强大。在此，我们开发了一种比例荧光探针 TCFPB–H ₂ S _n，用于在体外和体内选择性检测 H ₂ S _n 。值得注意的是，TCFPB–H ₂ S_n表现出对H ₂ S _n的快速、灵敏和特异性检测性能，并具有出色的溶酶体靶向能力和聚集诱导发射（AIE）特性。更重要的是，TCFPB–H ₂ S _n是第一个成功实现急性溃疡性结肠炎内源性 H ₂ S _n成像的探针。