There is a growing interest for the mixotrophic cultivation of microalgae using sustainable natural carbon sources. Chitin from shrimp waste was hydrolyzed using chitinase from Trichoderma asperellum and was exploited for the mixotrophic cultivation of microalgae. Chitin saccharification was optimized using Box-Behnken design to produce 50.41% (w/w) of reducing sugars using chitin, 0.83% (w/v), chitinase, 2.92 (v/v), temperature, 40 °C and time, 4.48 h. The biomass productivity of Tetradesmus obliquus and Aphanocapsa sp. was promoted to 39.1 and 40.57 mg L⁻¹ day⁻¹ in the presence of 0.5 g L⁻¹ of N-acetylglucosamine oligomers (GlcNAc), which was estimated to be ∼1.9 and ∼2-folds higher than the autotrophic conditions, respectively. Modified Logistic kinetic model confirmed higher growth rates and shortened lag-time of the mixotrophic cultures. The lipid productivity of both microalgae species was >1.5-times higher than the autotrophic conditions. Nitrate removal from the culture medium was found to be an effective strategy to increase both lipid content and lipid productivity for T. obliquus, but this strategy was not effective for Aphanocapsa sp. Fatty acid analysis revealed an increase of saturated fatty acids under mixotrophic cultivation for both microalgae species. Additionally, several biodiesel evaluation parameters indicated superior characteristics for the mixotrophic culture compared to the autotrophic conditions. Accordingly, mixotrophic growth of microalgae using GlcNAc as a new, sustainable carbon and nitrogen source opens a new avenue for biofuel production with an effective waste conversion into valuable commodities.

使用可持续的天然碳源对微藻进行混合营养培养越来越受到关注。使用来自曲霉木霉的几丁质酶水解虾废料中的几丁质，并被用于微藻的混合营养培养。使用 Box-Behnken 设计优化几丁质糖化，使用几丁质 0.83% (w/v)、几丁质酶 2.92 (v/v)、温度 40 °C 和时间 4.48 产生 50.41% (w/w) 的还原糖H。Tetradesmus obliquus和Aphanocapsa sp.的生物量生产力。在 0.5 g L ^-1的N存在下，提高到 39.1 和 40.57 mg L ^-1天^-1-乙酰氨基葡萄糖低聚物（GlcNAc），估计分别比自养条件高约 1.9 倍和约 2 倍。改进的 Logistic 动力学模型证实了混合营养培养物的更高增长率和更短的滞后时间。两种微藻物种的脂质生产力比自养条件高 1.5 倍以上。发现从培养基中去除硝酸盐是增加T. obliquus脂质含量和脂质生产力的有效策略，但该策略对Aphanocapsa 无效。sp. 脂肪酸分析显示，在混合营养培养下，两种微藻物种的饱和脂肪酸增加。此外，几个生物柴油评估参数表明，与自养条件相比，混合营养培养具有优越的特性。因此，使用 GlcNAc 作为新的、可持续的碳和氮源的微藻混合营养生长为生物燃料生产开辟了一条新途径，可将废物有效转化为有价值的商品。