Porcine circovirus type 2 (PCV2) has caused large economic losses in the swine industry worldwide; therefore, research on relevant therapeutic medicines is still urgently needed. To define the relationship between histone acetylation and inflammation induced by PCV2, we investigated whether traditional Chinese medicinal polysaccharides could alleviate viral infection by regulating histone acetylation. In this study, Sophora subprostrate polysaccharide (SSP)-treated PCV2-infected murine splenic lymphocytes in vitro and murine spleen in vivo were used to explore the regulatory effects of SSP on inflammation and histone acetylation caused by PCV2. SSP at different concentrations significantly reduced the secretion levels of the proinflammatory cytokines TNF-α and IL-6, the activity of COX-2, the mRNA expression levels of TNF-α, IL-6, iNOS and COX-2 and the protein expression levels of iNOS and COX-2 but promoted the secretion and mRNA expression levels of IL-10. Furthermore, the different concentrations of SSP significantly regulated the activity of histone acetylase (HAT) and the mRNA expression of HAT1, increased the activity of histone deacetylase (HDAC) and the mRNA expression of HDAC1 and reduced the protein expression levels of Ac-H3 and Ac-H4. Overall, SSP inhibited inflammation in PCV2-infected murine splenic lymphocytes by regulating histone acetylation in vitro and in vivo, thus playing an important role in PCV2 infection.

猪圆环病毒 2 型 (PCV2) 已在全球养猪业造成巨大经济损失；因此，仍然迫切需要研究相关的治疗药物。为了确定组蛋白乙酰化与 PCV2 诱导的炎症之间的关系，我们研究了中药多糖是否可以通过调节组蛋白乙酰化来减轻病毒感染。在这项研究中，苦参subprostrate多糖（SSP）处理的PCV2感染小鼠脾淋巴细胞在体外和小鼠脾体内用于探索 SSP 对 PCV2 引起的炎症和组蛋白乙酰化的调节作用。不同浓度的SSP显着降低促炎细胞因子TNF-α和IL-6的分泌水平、COX-2的活性、TNF-α、IL-6、iNOS和COX-2的mRNA表达水平和蛋白表达iNOS 和 COX-2 的水平，但促进了 IL-10 的分泌和 mRNA 表达水平。此外，不同浓度的SSP显着调节组蛋白乙酰化酶(HAT)活性和HAT1 mRNA表达，增加组蛋白去乙酰化酶(HDAC)活性和HDAC1 mRNA表达并降低 Ac-H3 和 Ac-H4 的蛋白表达水平。总体而言，SSP 通过在体外和体内调节组蛋白乙酰化来抑制 PCV2 感染的小鼠脾淋巴细胞的炎症，从而在 PCV2 感染中发挥重要作用。