The present study highlights a hybrid approach to capture and convert carbon dioxide (CO₂) from exhaust gases using bovine carbonic anhydrase (CA) submerged into aqueous amine solvents. Particularly, the sterically hindered amines have unique properties to absorb CO₂ and also play a key role in wide range of industrial applications. In this work, the reaction kinetics of CO₂ were studied in the aqueous phase of sterically hindered amines (SHAs), 2-amino-2-hyroxymethyl-1,3-propanediol (AHPD) and 2-amino-2-methyl-1-propanol (AMP), mixed with bovine CA as test and without CA as control using the stopped flow technique. The results showed a significant improvement in the rate of reaction between SHAs (either aqueous AHPD or aqueous AMP) and CO₂ in the presence of CA_. Moreover, the absorption capacity of SHA for CO₂ and its initial absorption rate in presence of CA was significantly enhanced. The rate constants of pseudo first-order reaction of CO₂ reacting with AHPD and AMP (each 0.5 kmol/m³) with varied concentration of CA were determined at temperature range of 298- 353 K. The aqueous AHPD and AMP systems in the presence of CA and control retained 65% of absorption capacity after 5 repetitive cycles. Also, the amine solvent captured CO₂ was converted into calcium carbonate (CaCO₃) which is white powder materials. The synthesized CaCO₃ was characterized for its morphological and physicochemical properties using scanning electron microscopy (SEM) and Fourier transformer infrared spectroscopy (FTIR) etc. Overall, as the CA present is the amine solvent can efficiently convert the CO₂ and that can be removed easily in the form of CaCO₃ precipitates.

本研究重点介绍了一种使用浸入水性胺溶剂中的牛碳酸酐酶 (CA) 从废气中捕获和转化二氧化碳 (CO ₂ )的混合方法。特别是，位阻胺具有独特的吸收 CO _{2 的}性能，并且在广泛的工业应用中也发挥着关键作用。在这项工作中，研究了 CO ₂在空间位阻胺 (SHA)、2-氨基-2-羟甲基-1,3-丙二醇 (AHPD) 和 2-氨基-2-甲基-1 的水相中的反应动力学-丙醇 (AMP)，与牛 CA 混合作为测试，不使用 CA 作为使用停流技术的对照。结果表明 SHA（AHPD 水溶液或 AMP 水溶液）与 CO ₂之间的反应速率显着提高在 CA 的存在下_。此外，SHA对CO ₂的吸收能力及其在CA存在下的初始吸收率显着增强。在 298-353 K 的温度范围内测定了 CO ₂与 AHPD 和 AMP（各 0.5 kmol/m ³）与不同 CA反应的拟一级反应的速率常数。5 次重复循环后，CA 和对照保持 65% 的吸收能力。此外，胺溶剂捕获的CO ₂转化为碳酸钙(CaCO ₃ )，它是白色粉末材料。合成的CaCO ₃使用扫描电子显微镜 (SEM) 和傅里叶变换红外光谱 (FTIR)等对其形态和物理化学性质进行了表征。总体而言，由于存在的 CA 是胺溶剂，因此可以有效地转化 CO ₂并且可以以 CaCO ₃沉淀物的形式轻松去除。