To investigate the effects of nano-ammonium octamolybdate (nano-Mo) and Macleaya cordata extracts (MCE) on antioxidant capacity of grazing Nanjiang brown goats on natural prairies under Cu and Cd stress, a 2 × 2 factorial design was adopted in this test, and two factors were nano-Mo (0, 10 mg/kg) and MCE (0, 3000 mg/kg). 24 hm2 polluted grassland was used in this 30-day trial and was equally divided into twelve fenced units. A total of 36 Nanjiang brown goats (1 year old) with an average body weight (BW) of 40.9 ± 2.1 kg were used in this test. The dietary treatments were (1) CON group, basic diet; (2) Nano-Mo group, basic diet + 10 mg/kg added Mo (nano-Mo); (3) MCE group, basic diet + 3000 mg/kg added MCE; and (4) combined group, basic diet + 10 mg/kg added nano-Mo and 3000 mg/kg added MCE. Nano-Mo or combination of nano-Mo and MCE diets significantly decreased the Cu content in serum and the liver of grazing goats (P < 0.05) and increased the Fe and Mo contents in serum and the liver (P < 0.05). The supplementation of nano-Mo, MCE, and combined diets extremely increased the levels of blood Hb, RBC, and PCV (P < 0.05), as well as the activities of serum SOD, GSH-Px, CAT, and Cp (P < 0.05), and greatly decreased the blood WBC content (P < 0.05) and the serum MDA content (P < 0.05). In conclusion, the application of nano-Mo and/or MCE diets on contaminated grasslands changed the contents of mineral elements in serum and the liver of grazing goats, reduced oxidative stress, and improved antioxidant capacity. The combination of nano-Mo and MCE can alleviate the toxic damage of combined heavy metal contaminations.

中文翻译：

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纳米钼和马齿苋提取物提高了在铜和镉污染大草原上放牧的南江棕山羊的抗氧化能力。

为研究纳米八钼酸铵（nano-Mo）和马齿苋提取物（MCE）对天然草原南江棕山羊在Cu和Cd胁迫下抗氧化能力的影响，本试验采用2×2因子设计，两个因素是纳米钼（0, 10 mg/kg）和MCE（0, 3000 mg/kg）。在这个为期 30 天的试验中使用了 24 hm2 的污染草地，平均分为 12 个围栏单元。本次试验共有36只南江棕山羊（1岁），平均体重（BW）为40.9±2.1 kg。饮食治疗为（1）CON组，基础饮食；(2)纳米钼组，基础日粮+10mg/kg添加钼(nano-Mo)；(3) MCE组，基础饮食+3000 mg/kg添加的MCE；(4)联合组，基础日粮+10mg/kg添加纳米钼和3000mg/kg添加MCE。纳米钼或纳米钼与MCE复合日粮显着降低放牧山羊血清和肝脏中的Cu含量（P < 0.05），增加血清和肝脏中的Fe和Mo含量（P < 0.05）。纳米钼、MCE、复合日粮的补充显着提高了血Hb、RBC、PCV的含量（P < 0.05），以及血清SOD、GSH-Px、CAT、Cp的活性（P < 0.05）。 0.05），显着降低血WBC含量（P < 0.05）和血清MDA含量（P < 0.05）。总之，纳米钼和/或MCE日粮在受污染草地上的应用改变了放牧山羊血清和肝脏中矿物质元素的含量，降低了氧化应激，提高了抗氧化能力。纳米钼与MCE的结合可以减轻结合重金属污染的毒性损害。