The development of an Aptamer based biosensor for the selective detection of human epidermal growth factor receptor 2 (HER2) with high sensitivity and specificity was achieved. A screen-printed carbon electrode was used in the scope of this work. The HER2 Aptamer was immobilized via electrostatic adsorption on the surface of a screen-printed electrode, which was modified with Au Nanoparticles (~ 20 nm diameter) to support the Aptamer immobilization. The Aptasensor was extensively investigated using Cyclic voltammetry, Differential pulse voltammetry, Electrochemical impedance spectroscopy, Fourier transform infrared spectroscopy and Atomic force microscopy. The Aptasensor exhibits a fast response with a binding time of only 5 min and shows a log-linear response over a wide concentration range of 0.001—100 ng/mL. Moreover, it has high sensitivity and enhanced detection limit reaching 52.85 μA/ng/mL, and 0.001 ng/mL, respectively, with a relative standard deviation < 5%. The Aptasensor selectivity was studied by using different interfering substances, and the results demonstrate that the Aptasensor is efficient for the detection of HER2 with approximately 8% extent of the interference.

实现了基于适体的生物传感器的开发，用于选择性检测人表皮生长因子受体 2 (HER2)，具有高灵敏度和特异性。在这项工作的范围内使用了丝网印刷碳电极。HER2 适配体通过静电吸附固定在丝网印刷电极的表面，该电极用 Au 纳米颗粒（直径约 20 nm）修饰以支持适配体固定。使用循环伏安法、微分脉冲伏安法、电化学阻抗谱、傅里叶变换红外光谱和原子力显微镜对适体传感器进行了广泛的研究。Aptasensor 具有快速响应，结合时间仅为 5 分钟，并在 0.001-100 ng/mL 的宽浓度范围内显示对数线性响应。而且，它具有高灵敏度和增强的检测限，分别达到 52.85 μA/ng/mL 和 0.001 ng/mL，相对标准偏差 < 5%。使用不同的干扰物质研究了适体传感器的选择性，结果表明适体传感器对 HER2 的检测是有效的，干扰程度约为 8%。