Archives of Microbiology ( IF 2.3 ) Pub Date : 2021-09-21 , DOI: 10.1007/s00203-021-02568-0 Hemangi Ranade 1 , Priya Paliwal 1 , Debarati Pal 2 , Manali Datta 1
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This paper introduces a novel prototype for the removal of Pseudomonas from water samples. Bacterial cells have the tendency to get attracted towards specific chemicals (chemotaxis); a ‘honey-based trap’ (henceforth, addressed as ‘honey-trap’) strip was conceptualized by integrating a combination of serine, pseudomonas-specific chemoattractant and honey to attract and inhibit the bacteria in situ. Honey, a natural antimicrobial agent, has garnered the attention as an effective inhibitor for Pseudomonal biofilms and wound infections. Dipping serine side of the strip attracted bacteria towards honey-trap, whereby the porous nature of the strip facilitated the ‘trapping’ and subsequent diffusion of the bacterial cells towards honey-adsorbed end of the strip. This ‘honey-trap’ reportedly leads to the targeted elimination of Pseudomonas, hence facilitating its removal. The percentage efficacy of this ‘honey-trap’ device is 96% with a log reduction equivalent to 1.6 within a time frame of 2 h. Pseudomonas aeruginosa, although, not a natural contaminant of potable water, enters circulation due to improperly maintained plumbing fixtures and storage facilities. Honey-trap strip is an easy to use, biodegradable and cost-effective sustainable solution, and thus a scaled-up version of this device may enable substantial improvement in quality of potable water.
Graphic abstract
Schematics showing the preparation and working of the Pseudomonas Honey-trap. Serine as an attractant and honey as an inhibitor was absorbed on filter strips (HT) for use. The strip was dipped in culture from serine end. After different time period of incubation, difference in bacterial load was confirmed by measuring the electrical conductivity and OD600nm of the culture. Additionally, inhibitory effect of HS was confirmed by placing the strip incubated with culture on agar plates and differences in bacterial lawn were monitored. Removal of bacterial cells from the suspension was also confirmed using absorption spectroscopy.
中文翻译：
基于蜂蜜的假单胞菌捕集器：水消毒的可持续原型
本文介绍了一种用于从水样中去除假单胞菌的新原型。细菌细胞倾向于被特定的化学物质吸引（趋化性）；通过整合丝氨酸、假单胞菌特异性化学引诱剂和蜂蜜的组合来原位吸引和抑制细菌，从而将“蜂蜜陷阱”（以下称为“蜂蜜陷阱”）条带概念化。蜂蜜是一种天然抗菌剂，作为一种有效的假单胞菌抑制剂引起了人们的关注。生物膜和伤口感染。条带的丝氨酸侧浸渍将细菌引向蜂蜜阱，由此条带的多孔性质促进了细菌细胞向条带的蜂蜜吸附端的“捕获”和随后的扩散。据报道，这种“蜂蜜陷阱”可以有针对性地消除假单胞菌，从而促进其去除。这种“蜂蜜陷阱”设备的效率百分比为 96%，在 2 小时的时间范围内对数减少相当于 1.6。铜绿假单胞菌，虽然不是饮用水的天然污染物，但由于管道装置和存储设施维护不当而进入循环。捕蜜条是一种易于使用、可生物降解且具有成本效益的可持续解决方案，因此该设备的放大版本可以显着改善饮用水的质量。
图形摘要
示意图显示了假单胞菌捕蜜器的制备和工作。作为引诱剂的丝氨酸和作为抑制剂的蜂蜜被吸附在过滤条（HT）上以供使用。将条带从丝氨酸末端浸入培养物中。培养不同时间后，通过测量培养物的电导率和OD600nm来确认细菌负荷的差异。此外，通过将与培养物一起孵育的条带放置在琼脂平板上并监测细菌草坪的差异来确认 HS 的抑制作用。还使用吸收光谱法确认了从悬浮液中去除细菌细胞。
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