Selective removal of an organic compound in the coexistence of other constituents is a great challenge in separation and purification processes. In this work, bovine serum albumin (BSA)-stabilized iridium nanoparticles (IrNPs) were prepared via a facile one-step precipitation method. The resulting BSA–IrNPs were comprehensively characterized by TEM, XRD, XPS, UV–vis, FT-IR, and fluorescence spectroscopy as well as circular dichroism spectrometry. It was found that the nanoparticles with an average diameter of 3.6 nm were embedded in the aggregated protein matrix and the structure of the coating agent was maintained well on the surface of nanoparticles. The as-prepared nanozymes (BSA–IrNPs) exhibit strong peroxidase-like activity and can selectively catalyse the degradation of cationic compounds by H₂O₂ in the coexistence of other inorganic or organic substances at room temperature. Interestingly, the degradation of amino acids could be precisely controlled by adjusting the pH above or below their isoelectric points. The catalytic selectivity of BSA–IrNPs should be ascribed to the anchoring effect between the amidogen-containing molecules and BSA through electrostatic adsorption. The nanozyme also exhibits excellent reusability as it can be readily recycled from solution by static settlement or centrifugation. Therefore, BSA–IrNPs have great potential for the selective removal of cationic compounds and amino acids in a complex matrix.

中文翻译：

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具有通常电荷选择性过氧化物酶特性的蛋白质稳定的 Ir 纳米颗粒

在其他成分共存的情况下选择性去除有机化合物是分离和纯化过程中的一大挑战。在这项工作中，通过简便的一步沉淀法制备了牛血清白蛋白 (BSA) 稳定的铱纳米粒子 (IrNPs) 。通过 TEM、XRD、XPS、UV-vis、FT-IR 和荧光光谱以及圆二色光谱对得到的 BSA-IrNPs 进行了全面表征。发现平均直径为3.6 nm的纳米颗粒嵌入聚集的蛋白质基质中，并且涂层剂的结构在纳米颗粒表面保持良好。所制备的纳米酶（BSA-IrNPs）表现出很强的过氧化物酶样活性，可以选择性地催化H对阳离子化合物的降解。₂ O ₂在常温下与其他无机或有机物质共存。有趣的是，氨基酸的降解可以通过调节高于或低于其等电点的 pH 值来精确控制。BSA-IrNPs的催化选择性应归因于含氨基分子与BSA之间通过静电吸附的锚定效应。纳米酶还表现出出色的可重复使用性，因为它可以通过静态沉降或离心从溶液中轻松回收。因此，BSA-IrNPs 在选择性去除复杂基质中的阳离子化合物和氨基酸方面具有巨大潜力。