One-step hydrothermal synthesis of nitrogen-doped carbon dots as a super selective and sensitive probe for sensing metronidazole in multiple samples,Analytical Methods

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One-step hydrothermal synthesis of nitrogen-doped carbon dots as a super selective and sensitive probe for sensing metronidazole in multiple samples
Analytical Methods ( IF 2.7 ) Pub Date : 2021-09-03 , DOI: 10.1039/d1ay01009c
Jinyan Du ₁ , Chaofeng Wang ₁ , Pingchuan Yuan ₂ , Qin Shu ₁ , Na Xu ₁ , Ying Yang ₁ , Shuangqing Qi ₁ , Yin Ye ₁ , Changqing Zhu ₁

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A reliable, super selective and sensitive nitrogen-doped carbon dots (N-CDs) nanoprobe that can quantitatively and quickly detect the concentration of metronidazole (MTZ) in multiple samples was built. We first prepared the N-CDs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC·HCl) as the precursor via a green, facile one-step hydrothermal method. The as-synthesized N-CDs were characterized by a variety of analytical and spectroscopic techniques, which revealed excitation-dependent fluorescence behavior with the maximum excitation and emission wavelengths being 335 and 370 nm, respectively. Significantly, the fluorescence emission of N-CDs underwent initial quenching upon the addition of MTZ via the inner filter effect (IFE), indicating a prospective detection method for MTZ. The linear range for MTZ detection was 0.1–250 μM, and the corresponding limit of detection (LOD) and limit of quantification (LOQ) were calculated to be only 70 nM and 233.33 nM, respectively. Moreover, due to the negligible cytotoxicity and superior biocompatibility, the fabricated N-CDs show a promising prospect for detecting MTZ in living cells. In general, our proposed N-CDs-based fluorescence sensing platform possesses super low LOD and LOQ values, wide linear range, and satisfactory selectivity, and can be applied to the detection of MTZ in multiple real samples.

中文翻译：

氮掺杂碳点的一步水热合成作为一种超选择性和灵敏探针，用于检测多个样品中的甲硝唑

构建了一种可靠、超选择性和灵敏的氮掺杂碳点（N-CDs）纳米探针，可定量快速检测多个样品中的甲硝唑（MTZ）浓度。我们首先制备使用N-CDS ñ - - （3-二甲氨基丙基）ñ作为前体'-ethylcarbodiimide盐酸盐（EDC·HCl的）经由一个绿色，轻便一步法水热法。合成后的 N-CDs 通过各种分析和光谱技术进行表征，这些技术揭示了激发依赖的荧光行为，最大激发和发射波长分别为 335 和 370 nm。值得注意的是，通过加入 MTZ，N-CDs 的荧光发射经历了初始淬灭。内过滤效应（IFE），表明了一种对 MTZ 的前瞻性检测方法。MTZ 检测的线性范围为 0.1–250 μM，相应的检测限 (LOD) 和定量限 (LOQ) 分别计算为仅 70 nM 和 233.33 nM。此外，由于可以忽略不计的细胞毒性和优异的生物相容性，制造的 N-CDs 在检测活细胞中的 MTZ 方面显示出广阔的前景。总的来说，我们提出的基于 N-CDs 的荧光传感平台具有超低 LOD 和 LOQ 值、宽线性范围和令人满意的选择性，可应用于多个实际样品中的 MTZ 检测。

更新日期：2021-09-22

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