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Polymerase Chain Reaction in the Detection of miR-455-5p and Sphingosine-1 Phosphate Proteins in Cervical Carcinoma with the Help of Gold Nanoparticles-Based.
Journal of Biomedical Nanotechnology Pub Date : 2021-9-22 , DOI: 10.1166/jbn.2021.3138
Xinping Chen 1 , Heqiu Ruan 2 , Zhichao Ma 2 , Junjie Hu 2 , Weihua Xu 2 , Liyan Yin 1 , Shengmiao Fu 2
Affiliation  

This study aimed to detect miR-455-5p and S1PR1 proteins using nanoparticle-assisted polymerase chain reaction (nano-PCR) to determine their correlation with cervical carcinoma prognosis. To achieve this study's goals, we selected 48 cervical carcinoma patients between January 2014 to January 2016 and subjected them to the miR-455-5p test by nano-PCR. The collected samples were then divided into two groups based on miR-455-5p levels. We had four HeLa cell groups, one group as the control, and one group overexpressed the miR-455-5p protein. A third group was miR-455-5p silent, and a separate group overexpressed both the miR-455-5p and S1PR1 proteins. Results also proved that the nano-PCR had a higher sensitivity than RT-PCR, and patients with poor prognosis had lesser miR-455-5p levels. Similarly, high levels of miR-455-5 contributed to cancer cell apoptosis and migration inhibition by targeting S1PR1 expression negatively. These two biomarkers are therefore significantly related to the prognosis of cervical carcinoma patients.

中文翻译:

聚合酶链反应在金纳米粒子的帮助下检测宫颈癌中的 miR-455-5p 和鞘氨醇-1 磷酸蛋白。

本研究旨在使用纳米粒子辅助聚合酶链反应 (nano-PCR) 检测 miR-455-5p 和 S1PR1 蛋白,以确定它们与宫颈癌预后的相关性。为了实现本研究的目标,我们选择了 2014 年 1 月至 2016 年 1 月期间的 48 名宫颈癌患者,并通过纳米 PCR 对他们进行了 miR-455-5p 测试。然后根据 miR-455-5p 水平将收集的样品分为两组。我们有四个 HeLa 细胞组,一组作为对照,一组过度表达 miR-455-5p 蛋白。第三组是 miR-455-5p 沉默,另一组过表达 miR-455-5p 和 S1PR1 蛋白。结果还证明,nano-PCR 比 RT-PCR 具有更高的灵敏度,并且预后较差的患者的 miR-455-5p 水平较低。相似地,高水平的 miR-455-5 通过消极地靶向 S1PR1 表达促进癌细胞凋亡和迁移抑制。因此,这两种生物标志物与宫颈癌患者的预后显着相关。
更新日期:2021-09-22
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