Theory of Active Intracellular Transport by DNA Relaying,Physical Review Letters

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Theory of Active Intracellular Transport by DNA Relaying
Physical Review Letters ( IF 8.1 ) Pub Date : 2021-09-21 , DOI: 10.1103/physrevlett.127.138101
Christian Hanauer ₁ , Silke Bergeler ₁ , Erwin Frey ₁ , Chase P Broedersz _{1,

2}

Affiliation

The spatiotemporal organization of bacterial cells is crucial for the active segregation of replicating chromosomes. In several species, including Caulobacter crescentus, the ATPase ParA binds to DNA and forms a gradient along the long cell axis. The ParB partition complex on the newly replicated chromosome translocates up this ParA gradient, thereby contributing to chromosome segregation. A DNA-relay mechanism—deriving from the elasticity of the fluctuating chromosome—has been proposed as the driving force for this cargo translocation, but a mechanistic theoretical description remains elusive. Here, we propose a minimal model to describe force generation by the DNA-relay mechanism over a broad range of operational conditions. Conceptually, we identify four distinct force-generation regimes characterized by their dependence on chromosome fluctuations. These relay force regimes arise from an interplay of the imposed ParA gradient, chromosome fluctuations, and an emergent friction force due to chromosome-cargo interactions.

中文翻译：

DNA 中继的细胞内主动转运理论

细菌细胞的时空组织对于复制染色体的主动分离至关重要。在几个物种中，包括Caulobacter crescentus，ATPase ParA 与 DNA 结合并沿细胞长轴形成梯度。新复制的染色体上的 ParB 分区复合体向上转移了这个 ParA 梯度，从而促进了染色体分离。一种源自波动染色体弹性的 DNA 中继机制已被提议作为这种货物易位的驱动力，但机械理论描述仍然难以捉摸。在这里，我们提出了一个最小模型来描述 DNA 中继机制在广泛的操作条件下产生的力。从概念上讲，我们确定了四种不同的力生成机制，其特征在于它们依赖于染色体波动。这些接力机制源于施加的 ParA 梯度、染色体波动、

更新日期：2021-09-21

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